33S solid-state NMR analysis of methionine radical inducing cytotoxicity of amyloid-beta protein

2013 Fiscal Year Final Research Report

• Project/Area Number: 24780108
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Tohoku University
• Principal Investigator: MASUDA YUICHI 東北大学, 薬学研究科(研究院), 助教 (90617755)
• Co-Investigator(Renkei-kenkyūsha): TAKEGOSHI Kiyonori 京都大学, 大学院理学研究科, 教授 (10206964) ; MURAKAMI Kazuma 京都大学, 大学院農学研究科, 准教授 (80571281)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 有機化学 / アミロイド / 固体NMR / 硫黄 / ラジカル

Research Abstract

We labeled sulfur atoms of methionine, cysteine and amyloid-beta with 33S by organic synthesis. We detected 33S NMR signals of methionine in solid-state and methionine sulfoxide in solution.

Research Products

• [Journal Article] Exploring various modulation-sideband recoupling conditions of SHA+ sequence at fast MAS. Solid State Nucl (2013)
  • Author(s): Shen, M., Liu, Q., Trébosc, J., Lafon, O., Masuda, Y., Takegoshi, K., Amoureux, J. P., Hu, B., Chen, Q.
  • Journal Title: Magn. Reson. Volume: 55–56 Pages: 42–47
  • DOI: 10.1016/j.ssnmr.2013.07.001
  • Peer Reviewed
• [Journal Article] Site-specific inhibitory mechanism for amyloid-β42 aggregation by catechol-type flavonoids targeting the Lys residues (2013)
  • Author(s): Sato, M., Murakami, K., Uno, M., Nakagawa, Y., Katayama, S., Akagi, K., Masuda, Y., Takegoshi, K., Irie, K.
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 23212-23224
  • DOI: 10.1074/jbc.M113.464222
  • Peer Reviewed
• [Journal Article] Solid-state NMR analysis of theβ-strand orientation of the protofibrils of amyloidβ-protein Biochem (2012)
  • Author(s): Doi, T., Masuda, Y., Irie, K., Akagi, K., Monobe,Y., Imazawa, T., Takegoshi, K.
  • Journal Title: Biophys. Res. Commun. Volume: 428 Pages: 458-462
  • DOI: 10.1016/j.bbrc.2012.10.096
  • Peer Reviewed
• [Journal Article] Very-long-distance correlations in proteins revealed by solid-state NMR spectroscopy (2012)
  • Author(s): Hu, B., Trebosc, J., Lafon, O., Chen, Q., Masuda, Y., Takegoshi, K., Amoureux, J. P.
  • Journal Title: ChemPhysChem Volume: 13 Pages: 3585-3588
  • DOI: 10.1002/cphc.201200548
  • Peer Reviewed
• [Presentation] タンパク質の高感度^<33>S-NMR解析に向けた^<33>S標識アミノ酸の合成 (2013)
  • Author(s): 増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 横浜(口頭発表, 講演番号29N-am09)
  • Year and Date: 2013-03-29
• [Presentation] ^<33>S標識によるタンパク質の高感度33S-NMR解析に向けて (2013)
  • Author(s): 増田裕一,松尾真嗣,水野敬,野田泰斗,竹腰清乃理,土井隆行
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台(口頭発表, 講演番号4C26a03)
  • Year and Date: 2013-03-27
• [Presentation] Solid-state NMR analysis of interaction sites of curcumin and amyloidβ-protein fibrils (2012)
  • Author(s): Masuda, Y., Fukuchi, M., Yatagawa, T., Tada, M., Takeda, K., Irie, K., Akagi, K., Monobe, Y., Imazawa, Y., Takegoshi, K.
  • Organizer: IKCOC-12
  • Place of Presentation: Kyoto(口頭発表, Program Number OP-21)
  • Year and Date: 2012-11-14
• [Presentation] Specific Inhibitory Mechanism of Aggregation of 42-mer Amyloid-βPeptide by Flavonoids (2012)
  • Author(s): Sato, M., Murakami, K., Ikubo, H., Uno, M., Nakagawa, Y., Katayama, S., Akagi, K., Masuda, Y., Takegoshi, K., Irie, K.
  • Organizer: IKCOC-12
  • Place of Presentation: Kyoto(ポスター発表, Poster Number PA-140)
  • Year and Date: 2012-11-13
• [Presentation] Cryocoil MASプローブによる固体有機物の^<33>S NMR測定にむけて (2012)
  • Author(s): 松尾真嗣,水野敬,野田泰斗,増田裕一,竹腰清乃理
  • Organizer: 第51回NMR討論会
  • Place of Presentation: 愛知(ポスター発表,講演番号P-81,査読なし)
  • Year and Date: 2012-11-10
• [Presentation] カテコール系フラボノイド類によるアミロイドβの凝集抑制機構 (2012)
  • Author(s): 村上一馬,佐藤瑞穂,井久保遥子,宇野真弓,中川優,片山寿美枝,赤木謙一,増田裕一,竹腰清乃理,入江一浩
  • Organizer: 第54回天然有機化合物討論会
  • Place of Presentation: 東京(口頭発表,講演番号17,査読あり)
  • Year and Date: 2012-09-19
• [Book] 遺伝子医学MOOK第21巻「最新ペプチド合成技術とその創薬研究への応用」(木曽良明編) (2012)
  • Author(s): 村上一馬,増田裕一,入江一浩
  • Total Pages: 211-216
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~hannou/index.html