2013 Fiscal Year Final Research Report
Functional design of glycoclusters possessing cross-linking activities with lectin
| Project/Area Number |
24780111
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Fukushima National College of Technology |
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Principal Investigator |
OGATA Makoto 福島工業高等専門学校, その他部局等, 助教 (10532666)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 分子認識 / 糖鎖工学 |
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| Research Abstract |
We are interested in developing an efficient synthetic route to multivalent glycosides, for glycomimetics, as they tend to have enhanced avidity due to their multivalency for specific lectins. In this study, various types of glycoclusters carrying alpha-2,6-sialyllactosamine, N-acetyllactosamine and Lewis X were designed and synthesized as glycomimetices. The interaction between each glycoclusters and three types lectins [Sambucus sieboldiana agglutinin (SSA), coral tree (Erythrina cristagalli) agglutinin (ECA) and Aleuria aurantia lectin (AAL)] is characterized using by hemagglutination inhibition assay, precipitation assay, isothermal titration calorimetry and dynamic light scattering.
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[Journal Article] Facile synthesis of sulfated sialoglycopolypeptides with aγ-polyglutamic acid backbone as hemagglutinin inhibitors against influenza virus2014
Author(s)
Ogata, M., Uzawa, H., Hidari, K. I., Suzuki, T., Park, E. Y., Usui, T.
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Journal Title
J . Appl. Glycosci.
Volume: 61
Pages: 1-7
Peer Reviewed
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[Journal Article] Sensitive and direct detection of receptor binding specificity of highly pathogenic avian influenza A virus in clinical samples2013
Author(s)
Takahashi, T., Kawakami, T., Mizuno, T., Minami, A., Uchida, Y., Saito, T., Matsui, S., Ogata, M., Usui, T., Sriwilaijaroen, N., Hiramatsu, H., Suzuki, Y., Suzuki, T.
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Journal Title
PLoS ONE
Volume: 18
Pages: e78125
Peer Reviewed
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[Journal Article] Expression and purification of bioactive hemagglutinin protein of highly pathogenic avian influenza A (H5N1) in silkworm larvae2013
Author(s)
Dong, J., Harada, M., Yoshida, S., Kato, Y., Murakawa, A., Ogata, M., Kato, T., Usui, T., Park, E. Y.
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Journal Title
J . Virol. Methods
Volume: 194
Pages: 271-276
Peer Reviewed
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[Journal Article] A novel transition-state analogue for lysozyme, 4-O-β-tri-N-acetylchitotriosyl moranoline, provided evidence supporting the covalent glycosyl-enzyme intermediate2013
Author(s)
Ogata, M., Umemoto, N., Ohnuma, T., Numata, T., Suzuki, A., Usui, T., Fukamizo, T.
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Journal Title
J . Biol. Chem.
Volume: 288
Pages: 6072-6082
Peer Reviewed
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[Journal Article] Enzymatic synthesis of cellulose II-like substance via cellulolytic enzyme-mediated transglycosylation in an aqueous medium.2012
Author(s)
Hattori, T., Ogata, M., Yumiko, K., Totani, K., Nikaido, M., Nakamura, T., Koshino, H., Usui, T.
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Journal Title
Carbohydr. Res.
Volume: 353
Pages: 22-26
Peer Reviewed
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[Journal Article] Facile synthesis of 4-O-β-N-acetylchitooligosyl 2-acetamido-2,3-dideoxydidehydro-gluconol actone based on transformation of chitooligosaccharide and its suppressive effects against the furylfuramide-induced SOS response2012
Author(s)
Ogata, M., Takeuchi, R., Suzuki, A., Hirai, H., Usui, T.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 76
Pages: 1362-1366
Peer Reviewed
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[Presentation] レクチンとの架橋複合体形成能を有する四価シアロ型糖鎖配位体の機能設計2012
Author(s)
尾形慎,矢野恵美,梅村征一郎,村田健臣,朴龍洙,小林夕香,浅井知浩,奥直人,中村直樹,松尾一郎,碓氷泰市
Organizer
第10回若手の力フォーラム
Place of Presentation
静岡県立大学(静岡県)
Year and Date
2012-09-06
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