2013 Fiscal Year Final Research Report
Role of Rpf in resuscitation from VBNC state in Salmonella
| Project/Area Number |
24780119
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Food science
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine |
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Principal Investigator |
KUSUMOTO Akiko 帯広畜産大学, 動物・食品検査診断センター, 助教 (60535326)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | VBNC / VNC |
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| Research Abstract |
To develop detection method for viable but non-culturable (VBNC) bacteria, we investigated molecular mechanism of VBNC state. For analysis of cell cycle of Salmonella, visualization method of DNA replication origin by FISH was established. While 1 or 2 foci of replication origin was detected in log phase cells, we failed to stain replication origin in VBNC cells. In VBNC state, physical state of chromosomal DNA might be different from that in log phase. Expression levels of FtsZ protein, which forms cell division ring, were not significantly different between stationary, log phase, and VBNC state. This indicates that VBNC state is not presumably induced by cell cycle arrest by reduction of FtsZ expression levels.
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