Evaluation of the effects of prebiotics on murine gut microbial ecology by multi-omic based network analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780133
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Keio University
• Principal Investigator: NAKANISHI Yumiko 慶應義塾大学, 政策・メディア研究科, 研究員 (10614274)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: メタボローム / 腸内細菌 / プレバイオティクス

Outline of Final Research Achievements

Raffinose is a non-digestive prebiotic oligosaccharide that has been reported to significantly increase the number of beneficial gut microbiota, such as Bifidobacterium. The purpose of this study is to evaluate the effects of raffinose dietary supplementation on murine gut microbial ecology using mass spectrometry-based metabolome analysis and analysis of fecal microbial composition using 16S rRNA sequencing. We analyzed male 8-weeks-old BALB/c mice that were fed a diet containing 5% raffinose for a week. Murine fecal metabolites were dramatically changed during after intake of raffinose supplemented diet, especially amino acids were significantly increased, indicating that amino acids might be play a role in prebiotic effects of raffinose degradation and fermentation.

Free Research Field

メタボロミクス