Physiological functions of fish glycoconjugates and their related enzymes

2015 Fiscal Year Final Research Report

• Project/Area Number: 24780204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Fisheries chemistry
• Research Institution: Kagoshima University
• Principal Investigator: Shiozaki Kazuhiro 鹿児島大学, 農水産獣医学域水産学系, 准教授 (70390896)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: メダカ / 糖脂質 / 糖タンパク / シアル酸 / シアリダーゼ

Outline of Final Research Achievements

Four medaka sialidase genes (neu1, neu3a, neu3b and neu4)has been cloned and characterized. Neu1 and Neu4 were localized at lysosome and their good substrates were oligosaccharides. On the other hand,　ganglioside　sialidases　Neu3a　and　Neu3b　were　detected　at　plasma　membrane　and　cytosol,　respectively.
Physiological functions of medaka sialidases were quite different from each other. Neu3a enhanced the differentiation of neurite formation. Neu3b positively regulated myoblast differentiation through the down-regulation of egfr mRNA expression. Neu1 and Neu4 are involved in the catabolism of glycoproteins in lysosome.

Free Research Field

水圏糖鎖生物学