Studies on screening, primary structural analysis, and mode of action of novel peptide toxins in deep-sea anemones

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780205
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Fisheries chemistry
• Research Institution: Tokai University Junior College
• Principal Investigator: HONMA Tomohiro 東海大学短期大学部, その他部局等, 准教授 (90435272)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 深海産イソギンチャク / オヨギイソギンチャク / ペプチド毒 / サワガニ / Naチャネル毒 / Kチャネル毒 / PaTX / 構造活性相関

Outline of Final Research Achievements

Seven novel peptide toxins from five species of deep-sea anemones, and two novel peptide toxins from one species of shallow-water sea anemones were isolated and their primary structures were analyzed. Furthermore, type 3 sodium channel peptide toxins of sea anemones were focused on because structure-activity relationships of the type 3 had not been clarified thoroughly, compared to type 1 and 2. Amino acid residues, which were considered to be essential for function of type 3 toxins, were specified. These studies revealed that many useful novel peptide toxins, which are not clarified yet, exist in the deep-sea anemones.

Free Research Field

水産化学