Exploring novel paternally methylated imprinted genes using bi-maternal mouse fetuses

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780265
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: Hokkaido University
• Principal Investigator: KAWAHARA manabu 北海道大学, (連合)農学研究科(研究院), 准教授 (70468700)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 個体発生 / インプリント遺伝子 / 核移植 / 多型解析 / 卵子ゲノム

Outline of Final Research Achievements

Parental genome functions in ontogeny are determined by interactions among transcripts from the maternal and paternal genomes. Comprehensive recognition of the interactions between parental genomes is important for understanding genomic imprinting in mammalian development. The bi-maternal (BM) embryos were constructed by only oocyte genomes, and therefore, analyses using those embryos enable us to investigate unknown paternally methylated imprinted genes in mouse genome. Comparing transcriptome between wild type (WT) and BM fetuses revealed that Mbnl1 expression in BM those was significantly reduced. We further examined Mbnl1 expression in uniparental fetuses, in which we found that Mbnl1 gene was downregulated in both parthenogenetic and androgenetic fetuses comparing that in WT fetus. These results suggested that Mbnl1 was non-imprinted gene but regulated by imprinted genes during development.

Free Research Field

家畜繁殖学