The role of neurokinin B and dynorphin A in GnRH pulse generation

2013 Fiscal Year Final Research Report

• Project/Area Number: 24780275
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied animal science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: YAMAMURA Takashi 独立行政法人農業生物資源研究所, 動物生産生理機能研究ユニット, 任期付研究員 (60582723)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: パルス状分泌 / ニューロキニン / キスペプチン / GnRH / マイクロダイアリシス / ヤギ

Research Abstract

It is suggested that neurokinin B (NKB) and dynorphin A (Dyn), which are coexpressed in kisspeptin neurons in the arcuate nucleus (ARC), are involved in gonadotrophin releasing hormone (GnRH) pulse generation. To clarify secretion patterns of NKB and Dyn in ARC, we developed in vivo microdialysis method and measured NKB and Dyn in ARC using goats. We enabled measurement of NKB concentration in ARC and observed fluctuations of NKB levels. Although the concentration fluctuations of NKB were not correlated well with pulsatile GnRH/luteinizing hormone release, these results suggested NKB could be secreted by kisspeptin neurons in ARC and involved in GnRH pulse generation.

Research Products

• [Journal Article] Electrophysiological and morphological evidence for synchronized GnRH pulse generator activity among kisspeptin/neurokinin B/dynorphin A (KNDy) neurons in goats (2013)
  • Author(s): Wakabayashi Yoshihiro, Takashi Yamamura, Kohei Sakamoto, Yuji Mori, Hiroaki Okamura
  • Journal Title: Journal of Reproduction and Development Volume: Vol.59(1) Pages: 40-48
  • DOI: 10.1262/jrd.2012-136
  • Peer Reviewed
• [Journal Article] Kisspeptin as a master player in the central control of reproduction in mammals : An overview of kisspeptin research in domestic animals (2013)
  • Author(s): Hiroaki Okamura, Takashi Yamamura, Yoshihiro Wakabayashi
  • Journal Title: Animal Science Journal Volume: vol.84 (5) Pages: 369-381
  • DOI: 10.1111/asj.12056
  • Peer Reviewed
• [Journal Article] A population of kisspeptin/neurokinin B neurons in the arcuate nucleus may be the central target of the male effect pheromone in goats (2013)
  • Author(s): Kohei Sakamoto, Yoshihiro Wakabayashi, Takashi Yamamura, Tomomi Tanaka, Yukari Takeuchi, Yuji Mori, Hiroaki Okamura
  • Journal Title: PLoS ONE Volume: 8(11) Pages: e81017
  • DOI: 10.1371/journal.pone.0081017
• [Presentation] 新規κオピオイド受容体拮抗剤の末梢投与がシバヤギGnRHパルスジェネレーター活動におよぼす効果 (2013)
  • Author(s): 伊藤太祐,中務桂佑,若林嘉浩,山村崇,岡村裕昭,大石真也,野口太朗,藤井信孝,上野山賀久,束村博子,前多敬一郎,松田二子,大蔵聡
  • Organizer: 第106回繁殖生物学会大会
  • Year and Date: 20130000
• [Presentation] NKBは弓状核キスペプチン/NKBニューロンに作用してGnRHパルスを産生させる (2012)
  • Author(s): 若林嘉浩,山村崇,大蔵聡,本間玲実,坂本光平,森裕司,岡村裕昭
  • Organizer: 第105回繁殖生物学会大会
  • Year and Date: 20120000
• [Presentation] Senktide, a neurokinin receptor agonist, stimulates pulsatile LH secretion through a mechanism mediated by the GnRH pulse generator in goats (2012)
  • Author(s): Yoshihiro Wakabayashi, Takashi Yamamura, Satoshi Ohkura, Tamami Homma, Kohei Sakamoto, Yuji Mori, Hiroaki Okamura
  • Organizer: 42nd Society for Neuroscience Annual Meeting
  • Year and Date: 20120000
• [Presentation] Structure-activity relationship study of tachykinin peptides for development of novel neurokinin-3 receptor agonists (2012)
  • Author(s): Ryosuke Misu, Shinya Oishi, Taro Noguchi, Ai Yamada, Hiroaki Ohno, Takashi Yamamura, Hiroaki Okamura, Fuko Matsuda, Satoshi Ohkura, Nobutaka Fujii
  • Organizer: 2nd World Conference on Kisspeptin Signaling in the Brain
  • Year and Date: 20120000
• [Presentation] Continuous administration of senktide, a neurokinin receptor agonist, facilitates intermittent activities of the GnRH pulse generator for 24h in goats (2012)
  • Author(s): Takashi Yamamura, Yoshihiro Wakabayashi, Taro Noguchi, Shinya Oishi, Nobutaka Fujii, Satoshi Ohkura, Hiroaki Okamura
  • Organizer: 2nd World Conference on Kisspeptin Signaling in the Brain
  • Year and Date: 20120000