2013 Fiscal Year Final Research Report
Novel influenza A virus proteins translated from PA mRNA
| Project/Area Number |
24780296
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied veterinary science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MURAMOTO Yukiko 東京大学, 医科学研究所, 特任研究員 (70436567)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | インフルエンザウイルス |
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| Research Abstract |
I identified novel PA-related proteins in influenza A virus-infected cells. These proteins are translated from the eleventh and thirteenth in-frame AUG codons in the PA mRNA and are, therefore, N-terminally truncated forms of PA, which we named PA-N155 and PA-N182, respectively. The eleventh and thirteenth AUG codons are highly conserved among influenza A viruses, and the PA-N155 and PA-N182 proteins were detected in cells infected with various influenza A viruses isolated from different host species, suggesting the expression of these N-truncated PAs is universal in nature among influenza A viruses. These N-truncated PAs did not show polymerase activity when expressed together with PB1 and PB2; however, mutant viruses lacking the N-truncated PAs replicated more slowly in cell culture and had lower pathogenicity in mice than did wild-type virus. These results suggest that these novel PA-related proteins likely possess important functions in the replication cycle of influenza A virus.
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Research Products
(8 results)
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[Journal Article] Integrated clinical, pathologic, virologic, and transcriptomic analysis of H5N1 influenza virus-induced viral pneumonia in the rhesus macaque2012
Author(s)
Shinya K, Gao Y, Cilloniz C, Suzuki Y, Fujie M, Deng G, Zhu Q, Fan S, Makino A, Muramoto Y, Fukuyama S, Tamura D, Noda T, Eisfeld AJ, Katze MG, Chen H, Kawaoka Y
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Journal Title
J Virol
Volume: 86
Pages: 6055-6066
DOI
Peer Reviewed
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