2013 Fiscal Year Final Research Report

Analysis of CDV quasispecies and establishement of the reverse genetics system

Research Project

Project/Area Number	24780303
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Applied veterinary science
Research Institution	National Institute of Infectious Diseases
Principal Investigator	YOSHIKAWA Tomoki 国立感染症研究所, ウイルス第一部, 研究員 (20399463)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	イヌジステンパーウイルス
Research Abstract	There was a canine distemper outbreak, which is generally known to be occurred in carnivore happened in the monkey colony in an animal quarantine. Hence to understand the pathogenesis, the sequences from the in vitro isolates and from these of clinical specimens were determined and analyzed by utilizing next-generation sequencing technique. In addition, establishment of the reverse genetics system based on the S-CDV sequence, which enable to produce the infectious particles from the plasmids were tried.