Elucidation of the molecular network controlling sperm acrosome formation in mammals

2013 Fiscal Year Final Research Report

• Project/Area Number: 24780327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Tsukuba
• Principal Investigator: KANEMORI Yoshinori 筑波大学, 生命環境系, 助教 (40529088)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: マウス / 精子形成 / 受精

Research Abstract

Mammalian sperm possess a cap-shaped, exocytotic vesicle, acrosome that is located at the anterior part of the nucleus. ACRBP/sp32, is an acrosomal protein, initially identified as a binding protein for the precursor (proACR) of sperm serine protease ACR. Unlike other mammalian ACRBPs, two forms of Acrbp mRNA, wild-type ACRBP-W and variant Acrbp-V5 mRNAs, were generated by alternative splicing of Acrbp in the mouse. In this study, we produced knockout mice lacking ACRBP to uncover the role of ACRBP in spermatogenesis and fertilization. These results demonstrate that ACRBP-V5 palys key role in condensation and subsequent spread of acrosomal proteins in the acrosome during spermiogenesis. It is also suggested that ACRBP-W may function in the retention of proACR in the acrosome until after sperm undergo acrosomal exocytosis.

Research Products

• [Journal Article] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in the mouse (2013)
  • Author(s): Kanemori, Y., Ryu, J. H., Sudo, M., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, S., and Baba, T.
  • Journal Title: Biol. Reprod. Volume: 88 Pages: 1-8
  • Peer Reviewed
• [Presentation] 精子アクロソームタンパク質ACRBPとACRのダブル欠損マウスの解析 (2013)
  • Author(s): 高嶋明日登、兼森芳紀、首藤舞、康宇鎮、馬場忠
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸国際会議場(ポスター)
  • Year and Date: 2013-12-04
• [Presentation] Proacrosin-binding protein ACRBP/sp32 regulates acrosome formation during mouse spermiogenesis. (2013)
  • Author(s): Kanemori, Y., Sudo, M., Koga, Y., Okabe, M., and Baba, T.
  • Organizer: Gordon Research Conference Program on Fertilization & Activation of Development
  • Place of Presentation: Holderness School Holderness, NH, USA (ポスター発表)
  • Year and Date: 2013-07-15
• [Presentation] Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in mouse spermatogenesis (2013)
  • Author(s): Sudo, M., Kanemori, Y., Ryu, JH., Niida-Araida, Y., Kodaira, K., Takenaka, M., Kohno, N., Sugiura, S., Kashiwabara, SI., and Baba, T.
  • Organizer: Gordon Research Conference Program on Fertilization & Activation of Development
  • Place of Presentation: Holderness School Holderness, NH, USA (ポスター発表)
  • Year and Date: 2013-07-15
• [Presentation] Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization (2012)
  • Author(s): 柳辰協、首藤舞、兼森芳紀、柏原真一、馬場忠
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(ポスター発表)
  • Year and Date: 2012-12-14
• [Remarks]
  • URL: http://www.acroman.org/