Establishment of innovative angiognenic therapy using with in vivo imaging of angiogenesis

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790038
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: HAMADA Yoh 東北大学, 医学(系)研究科(研究院), 助教 (20611958)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 血管新生治療 / 血管新生メカニズム

Outline of Final Research Achievements

To establish double imaging technique which can visualize vascular endothelial and mural cells together, we intend to make novel hetero-tagged recombinant VEGF and PDGF. Finally, we succeeded in establishing these proteins. At first, we checked these normal physiological activity. Cells which expressed these receptors snowed significantly high fluorescence with protein-fluorescent particle complex.
Then, we tried to visualize vascular endothelial and mural cells simultaneously using with animal model. But, there were several difficulty such as auto-fluorescence. It took too much time to decide adequate imaging condition. Moreover, we have much trouble to make virus particles for angiogenic therapy. So, we could not accomplish our aim enough. We want to continue making effort to overcome several problems which we confront.

Free Research Field

血管新生