Glycomics for identification of rotavirus receptor and development of antiviral agent

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Kinki University
• Principal Investigator: YAMADA Keita 近畿大学, 薬学部, 研究員 (80584185)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ロタウイルス / 硫酸化糖鎖 / N-グリコリルノイラミン酸 / 糖脂質 / 糖鎖アレイ / 分析化学

Research Abstract

Rotavirus is the major etiology of severe gastroenteritis in infants especially in developing countries. It is indicated that human rotavirus (hRV) recognizes glycans expressed on host cells as the initial step of virus-cell entry. In this study, glycan structures recognized by hRV were studied by the glycan array method, which we previously established. We found that hRV (MO strain) strongly recognizes NeuGc residues and sulfated glycans. These results indicated that NeuGc and sulfated glycans show an important role in human rotavirus infection.

Research Products

• [Journal Article] Bovineκ-casein inhibits human rotavirus (HRV) infection via direct binding of glycans to HRV (2014)
  • Author(s): Inagaki M, Muranishi H, Yamada K, Kakehi K, Uchida K, Suzuki T, Yabe T, Nakagomi T, Nakagomi O, Kanamaru Y
  • Journal Title: Journal of dairy science Volume: 97(5) Pages: 2653-61
  • DOI: 10.3168/jds.2013-7792
  • Peer Reviewed
• [Journal Article] Common glycoproteins expressing polylactosamine-type glycans on matched patient primary and metastatic melanoma cells show different glycan profiles (2014)
  • Author(s): Kinoshita M, Mitsui Y, Kakoi N, Yamada K, Hayakawa T, Kakehi K
  • Journal Title: Journal of Proteome Research Volume: 13(2) Pages: 1021-33
  • DOI: 10.1021/pr401015b
  • Peer Reviewed
• [Journal Article] Analysis of O-glycans as 9-fluorenylmethyl derivatives and its application to the studies on glycan array (2013)
  • Author(s): Yamada K, Hirabayashi J, Kakehi K
  • Journal Title: Analytical chemistry Volume: 85(6) Pages: 3325-33
  • DOI: 10.1021/ac303771q
  • Peer Reviewed
• [Journal Article] Free glycans derived from glycoproteins present in human sera (2013)
  • Author(s): Iwatsuka K, Watanabe S, Kinoshita M, Kamisue K, Yamada K, Hayakawa T, Suzuki T, Kakehi K
  • Journal Title: Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences Volume: 928 Pages: 16-21
  • DOI: 10.1016/j.jchromb.2013.03.010
  • Peer Reviewed
• [Journal Article] Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells (2012)
  • Author(s): Mitsui Y, Yamada K, Hara S, Kinoshita M, Hayakawa T, Kakehi K
  • Journal Title: Journal of Pharmaceutical and Biomedical Analysis Volume: 70 Pages: 718-26
  • DOI: 10.1016/j.jpba.2012.06.035
  • Peer Reviewed
• [Journal Article] Hypersialylated type-I lactosamine-containing N-glycans found in Artiodactyla sera are potential xenoantigens (2012)
  • Author(s): Sumiyoshi W, Nakakita S, Miyanishi N, Yamada K, Hasehira K, Nakakita Y, Hirabayashi J
  • Journal Title: Glycobiology Volume: 22(8) Pages: 1031-41
  • DOI: 10.1093/glycob/cws069
  • Peer Reviewed
• [Presentation] 癒着防止剤中カルボキシメチルセルロースの構造評価技術 (2014)
  • Author(s): 山田佳太,木下充弘,徐吉夫,井上利樹,青島元法,長谷川清孝,清一雄,北荘一郎,掛樋一晃
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-29
• [Presentation] ロタウイルスエントリー機構の解明に向けたグライコミクス (2014)
  • Author(s): 山田佳太,栢原春奈,稲垣瑞穂,木下充弘,金丸義敬,鈴木徹,矢部富雄,中込とよ子,中込治,掛樋一晃
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本
  • Year and Date: 2014-03-28
• [Presentation] 牛乳由来タンパク質ラクトフォリンの感染阻害作用に関する研究 (2013)
  • Author(s): 大野翔平,稲垣瑞穂,山田佳太,鈴木徹,松田幹,高橋毅,杉山誠,中込とよ子,中込治,矢部富雄,金丸義敬
  • Organizer: 日本農芸化学会中部支部第168回例会
  • Place of Presentation: 愛知
  • Year and Date: 2013-10-12
• [Presentation] ヒトロタウイルス感染機構の解明に向けたグライコミクス解析 (2013)
  • Author(s): 山田佳太,栢原春奈,稲垣瑞穂,金丸義敬,矢部富雄,鈴木徹,中込治,中込とよ子,木下充弘,掛樋一晃
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2013-08-05
• [Presentation] ヒトロタウイルスが認識する糖鎖の解析とその臨床化学的応用の可能性 (2013)
  • Author(s): 山田佳太,栢原春奈,稲垣瑞穂,金丸義敬,鈴木徹,中込治,中込とよ子,木下充弘,早川堯夫,掛樋一晃
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 神奈川
  • Year and Date: 2013-03-29
• [Presentation] 多様なムチン型糖鎖アレイの作製を可能にする糖鎖ライブラリー構築技術 (2013)
  • Author(s): 山田佳太,平林淳,木下充弘,早川堯夫,掛樋一晃
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: 神奈川
  • Year and Date: 2013-03-29
• [Presentation] ヒトロタウイルスは,牛乳カッパカゼインの糖鎖構造を認識する (2012)
  • Author(s): 稲垣瑞穂,村西晴香,山田佳太,内田健志,鈴木徹,矢部富雄,中込とよ子,中込治,金丸義敬
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-14
• [Presentation] 牛乳ラクトフォリンの示すヒトロタウイルス感染阻害作用(続報) (2012)
  • Author(s): 大野翔平,稲垣瑞穂,山田佳太,矢部富雄,鈴木徹,高橋毅,杉山誠,中込とよ子,中込治,金丸義敬
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2012-11-14
• [Presentation] Fmoc標識化法を用いたO-結合型糖鎖解析-汎用性の高いO-結合型糖鎖ライブラリーの構築- (2012)
  • Author(s): 山田佳太、平林淳、掛樋一晃
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-09-19
• [Presentation] ヒト胃腺癌細胞MKN45細胞は糖タンパク質由来遊離糖鎖を細胞外へ分泌する (2012)
  • Author(s): 神末和哉,大河原周平、山田佳太、岩塚欣也,木下充弘,早川堯夫,掛樋一晃
  • Organizer: 第31回日本糖質学会年会
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-09-18
• [Presentation] The inhibitory mechanism of bovine lactophorin against human rotavirus gastroenteritis (2012)
  • Author(s): Mizuho Inagaki, Shohei Ohno, Keita Yamada, Takeshi Takahashi, Yoshitaka Nakamura, Tsukasa Matsuda, Tomio Yabe, Tohru Suzuki, Makoto Sugiyama, Toyoko Nakagomi, Osamu Nakagomi, and Yoshihiro Kanamaru
  • Organizer: World Congress of Microbes-2012
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2012-07-30