Structure determination and Characterization of proline-specific aminopeptidases

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790057
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Hyogo University of Health Sciences
• Principal Investigator: NAKANO Hiroaki 兵庫医療大学, 薬学部, 助教 (10378789)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: X線結晶解析 / X線溶液散乱 / 宇宙実験 / タンパク質 / コラーゲン / 基質認識

Research Abstract

Proline-specific aminopeptidases are critical to the degradation of proline-rich peptides and proteins, such as collagen and its denatured form, gelatin. Collagens are the largest components in animal tissues and the most major hard-to-degrade proteins because of their unique fibrils composed of triple helical wound polypeptides. In this study, we crystallized three of the recombinant enzymes (Pz-A, Pz-B, and AM-1), and performed its preliminary X-ray crystallographic analysis. We have determined the entire structure of the these aminopeptidases, found a very unique apical domain sharing the interface with a domain containing a main active site and described about the characteristic structure and mechanism for the substrate recognition for proly peptides.

Research Products

• [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of Pz peptidase B from Geobacillus collagenovorans MO-1 (2012)
  • Author(s): Nakano, H., Hosokawa, A., Tagawa, R., Inaka, K., Ohta, K., Nakatsu, T., Kato, H., Watanabe, K
  • Journal Title: Acta Crystallographica Volume: F68 Pages: 757-759
  • DOI: 10.1107/S1744309112018969
  • Peer Reviewed
• [Presentation] 既存プロリダーゼとは異なるGly-Pro分解性システインペプチダーゼの生化学的解析と結晶化 (2014)
  • Author(s): 河野良輔、坂本琢馬、中野博明、渡部邦彦
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 明治大学生田キャンパス(神奈川県川崎市)
  • Year and Date: 20140327-30
• [Presentation] Study of a peptidase-associated domain of an aminopeptidase from thermophilic Aneurinibacillus sp. AM-1 (2013)
  • Author(s): Ryuji Tagawa, Hiroaki Nakano, Kunihiko Watanabe
  • Organizer: 第51回日本生物物理学会年会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 20131028-30
• [Book] タンパク質結晶の最前線 (2013)
  • Author(s): 杉山成、高野和文、喜多俊介、前仲勝実、福原秀雄、清水義宏、禾晃和、三原恵美子、高木淳一、秋葉宏樹、津本浩平、原利明、山口宏、吉川洋史、丸山美帆子、中野博明、渡部邦彦、松森信明、村上聡、名倉淑子、小笠原諭、田辺幹雄、橘勝、小島謙一、西澤典彦、松村浩由、塚本勝男、馬場清喜、熊坂崇、水野伸宏、溝端栄一、鈴木守、南後恵理子、田中里枝、井上豪、岩田想、濡木理、玉田太郎、川口充康、長野哲雄、多田幸雄、松岳大輔、中迫雅由、野村祐介、坂本泰一、川上勝、菅原道泰、国島直樹、仁田雅弘、三宅隆弘、澤匡明、安達宏昭
  • Total Pages: 担当部分p.84-91
  • Publisher: シーエムシー出版