2014 Fiscal Year Final Research Report
The development of the non-invasive redox measurement technique with liver-targeting liposomes.
| Project/Area Number |
24790058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Sojo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TAKESHITA Keizo 崇城大学, 薬学部, 教授 (70175438)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ニトロキシルラジカル / リポソーム / 電子スピン共鳴 / 酸化ストレス / レドックス |
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| Outline of Final Research Achievements |
In vivo redox status are involved in the various diseases. In vivo electron spin resonance (ESR) spectroscopy is a very useful technique for the measurement of in vivo redox status. The extension of half-life in the body and the tissue targeting of redox probes for in vivo ESR are important for developing the more precise in vivo redox measurement. Therefore, the purpose of this study is to make the liver-targeting liposomes labeled by redox probes.
The redox probe-conjugated phospholipids (DMPE-PROXYL) were synthesized. The liposome including the DMPE-PROXYL gave the ESR signal in vitro. The ESR signal of this liposome decreased by the reaction with hydroxyl radical, but did not decreased by ascorbic acid or liver homogenate. This suggests that the oxidative stress can be detected by the liposome including the DMPE-PROXYL.
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| Free Research Field |
生物物理
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