2013 Fiscal Year Final Research Report
Membrane localization of LRRK2, a causative gene for familial Parkinson's
| Project/Area Number |
24790068
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Genta 東京大学, 医学(系)研究科(研究員), 助教 (10431892)
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| Project Period (FY) |
2012 – 2013
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| Keywords | パーキンソン病 / LRRK2 / キナーゼ |
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| Research Abstract |
It has been reported that LRRK2 exists on membrane of organelles or vesicles in cytoplasm by electron microscopic analysis. However, the membrane localization of LRRK2 has not been precisely characterized. In this study, we found that asmall fraction of LRRK2 was eluted in the detergent-soluble membrane fraction. We also found that LRRK2 translocates to the LAMP1-positive structure upon treatment of cells with a LRRK2 inhibitor. In addition, we succeeded in forcing LRRK2 to translocate to organelles by using a chemical-induced localization system.
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