Establishment of evaluation methods for molecular targeted anticancer drugs targeting organelle specific stress response in the cell

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790072
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Kyoto University
• Principal Investigator: HORIBE Tomohisa 京都大学, 医学(系)研究科(研究院), 特定講師 (20467468)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 薬学 / 癌 / ストレス / タンパク質

Research Abstract

To establish the evaluation methods for molecular targeted anticancer drugs targeting organelle specific stress response in cancer cell, reporter vectors using promoter regions of responsive genes for organelle specific unfolded protein response (UPR) were constructed, and then assessed by luminometer and single-cell bioluminescence imaging system. It was confirmed that bioluminescence imaging system at single cell level was available tools for the evaluation methods using the constructed reporter vectors in this study.

Research Products

• [Journal Article] Transfection efficiency of normal and cancer cell lines and monitoring of promoter activity by single-cell bioluminescence imaging (2014)
  • Author(s): Horibe, T., Torisawa, A., Akiyoshi, R., Hatta-Ohashi, Y., Suzuki, H., and Kawakami, K
  • Journal Title: Luminescence Volume: 29 Pages: 96-100
  • DOI: 10.1002/bio.2508
• [Presentation] Increase of cytotoxic activity induced by Antp-TPR hybrid peptide in the presence of Hsp70-targeted peptide to breast cancer cells (2013)
  • Author(s): Horibe, T., Torisawa, A., Kohno, M., and Kawakami, K
  • Organizer: 第86回日本生化学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 20130911-13
• [Presentation] Functional analysis of cytotoxic activity to glioblastoma cells by Antp-TPR hybrid peptide targeting Hsp90 (2013)
  • Author(s): Horibe, T. and Kawakami, K
  • Organizer: 第17回日本がん分子標的治療学会
  • Place of Presentation: 京都国際会館
  • Year and Date: 20130612-14
• [Presentation] Molecular mechanism of cancer cell death by Antp-TPR hybrid peptide targeting Hsp90 in glioblastoma cells (2012)
  • Author(s): Horibe, T., Torisawa, A., Kohno, M., and Kawakami, K
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] Functional analysis of cytotoxic activity to acute myeloid leukemia cells by Antp-TPR hybrid peptide targeting Hsp90 (2012)
  • Author(s): Horibe, T., Kawamoto, M., Kohno, M., and Kawakami, K
  • Organizer: 第49回ペプチド討論会
  • Place of Presentation: 鹿児島県民交流センター
  • Year and Date: 20121107-09