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2013 Fiscal Year Final Research Report

Regulatory mechanism of Reelin proteolysis

Research Project

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Project/Area Number 24790081
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biological pharmacy
Research InstitutionNagoya City University

Principal Investigator

KOHNO Takao  名古屋市立大学, 薬学研究科(研究院), 助教 (70581742)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsリーリン / プロテアーゼ / プロテオリシス
Research Abstract

Reelin is a secreted glycoprotein that plays essential roles in the brain formation. Reelin is specifically cleaved at two sites (N-t and C-t site). However, the mechanism and the physiological role of Reelin cleavage is largely unknown. In this study, we determined the N-t cleavage site, and found that N-t cleavage of Reelin plays critical roles in regulating the duration and range of Reelin functions. Furthermore, we identified the protease from supernatant of cultured neurons, and found that this protease is the major protease in charge of Reelin cleavage in embryonic cerebral cortex.

  • Research Products

    (15 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (11 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Cleavage within Reelin Repeat 3 Regulates the Duration and Range of the Signaling Activity of Reelin Protein2014

    • Author(s)
      Koie M, Okumura K, Hisanaga A, Kamei T, Sasaki K, Deng M, Baba A, Kohno T and Hattori M
    • Journal Title

      J. Biol. Chem

      Volume: 289 Pages: 12922-12930

    • DOI

      10.1074/jbc.M113.536326.

    • Peer Reviewed
  • [Journal Article] A disintegrin and metalloproteinase with thrombospondin motifs 4 (ADAMTS-4) cleaves Reelin in an isoform-dependent manner2012

    • Author(s)
      Hisanaga A, Morishita S, Suzuki K, Sasaki K, Koie M, Kohno T and Hattori M.
    • Journal Title

      FEBS Lett

      Volume: 586 Pages: 3349-3353

    • DOI

      10.1016/j.febslet.2012.07.017.

    • Peer Reviewed
  • [Presentation] The mechanism and physiological significance of proteolytic cleavage of Reelin2013

    • Author(s)
      Arisa Hisanaga, Takao Kohno, Mitsuharu Hattori
    • Organizer
      Neuroscience2013 : Society for Neuroscience
    • Place of Presentation
      サンディエゴ
    • Year and Date
      2013-11-11
  • [Presentation] 精神神経疾患の治療を目指した、リーリンの特異的分解機構の解明2013

    • Author(s)
      河野孝夫
    • Organizer
      第57回日本薬学会関東支部大会
    • Place of Presentation
      帝京大学
    • Year and Date
      2013-10-26
  • [Presentation] リーリンの新規分解は、生後の神経発達を制御する2013

    • Author(s)
      河野孝夫、本田岳夫、久保健一郎、中野良美、村上達郎、仲嶋一範、服部光治
    • Organizer
      日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-09-11
  • [Presentation] 脳の形成と機能に重要な分泌タンパク質リーリンの切断機構の解明2013

    • Author(s)
      久永有紗、鯉江真利、奥村恭子、河野孝夫、服部光治
    • Organizer
      日本生化学会大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2013-09-11
  • [Presentation] リーリンの特異的分解の機構と生理的意義2013

    • Author(s)
      奥村恭子、久永有紗、鯉江真利、河野孝夫、服部光治
    • Organizer
      Neuro2013
    • Place of Presentation
      京都国際会館
    • Year and Date
      2013-06-21
  • [Presentation] 脳の形成を司る細胞外因子リーリンのプロテオリシスによる機能制御機構の解明2013

    • Author(s)
      河野孝夫
    • Organizer
      生理研研究会
    • Place of Presentation
      生理学研究所
    • Year and Date
      2013-06-07
  • [Presentation] 大脳皮質上層神経細胞の樹状突起配向におけるリーリンの新規機能2012

    • Author(s)
      河野孝夫、本田岳夫、久保健一郎、中野良美、村上達郎、仲嶋一範、服部光治
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      マリンメッセ福岡
    • Year and Date
      2012-12-15
  • [Presentation] 脳の形成と機能に重要な分泌タンパク質リーリンの切断機構の解明2012

    • Author(s)
      久永有紗、鯉江真利、河野孝夫、服部光治
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2012-12-11
  • [Presentation] Novel regulatory mechanism of the Reelin functions2012

    • Author(s)
      河野孝夫、本田岳夫、久保健一郎、村上達郎、中野良美、本間夏美、仲嶋一範、服部光治
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-21
  • [Presentation] Characterization of the protease in charge of specific cleavage of Reelin2012

    • Author(s)
      久永有紗、森下駿介、鯉江真利、河野孝夫、服部光治
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-20
  • [Presentation] The N-t site cleavage of Reelin occurs after specific residue2012

    • Author(s)
      鯉江真利、久永有紗、佐々木一友、森下駿介、河野孝夫、服部光治
    • Organizer
      第35回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2012-09-20
  • [Remarks]

    • URL

      http://www.phar.nagoya-cu.ac.jp/hp/bsk/indexj1.html

  • [Patent(Industrial Property Rights)] ADAMTS-3を用いたリーリン分解2012

    • Inventor(s)
      服部光治、河野孝夫、久永有紗、鯉江真利、鈴木健太
    • Industrial Property Rights Holder
      公立学校法人名古屋市立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特願2012-180354
    • Filing Date
      2012-08-16

URL: 

Published: 2015-06-25  

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