2014 Fiscal Year Final Research Report
Isolation and comprehensive analysisi of constituents in oxidized low-density lipoprotein in vivo
| Project/Area Number |
24790087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Showa University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 酸化LDL / 動脈硬化症 / リピドミクス / リポタンパク質 / モノクローナル抗体 |
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| Outline of Final Research Achievements |
Oxidized low-density lipoprotein (oxLDL) has been known as one of key factors for development of atherosclerosis. However, molecular features of in vivo oxLDL remain to be clarified. To characterize in vivo oxLDL, we performed an effective isolation of oxLDL from plasma followed by LC-ESI-MS/MS analysis of its lipid components. Combination of anion-exchange column separation of electronegative LDL (LDL(-)) and affinity purification using a monoclonal antibody against oxidized phosphatidylcholine (oxPC) revealed that oxLDL was enriched in LDL(-) fraction. In vivo oxLDL showed a different profile of PC molecular species from Cu-induced (in vitro) oxLDL. These data suggests that oxidized lipids in LDL may converted to other molecular species in vivo.
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| Free Research Field |
生化学
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