2013 Fiscal Year Final Research Report
Proteomic analysis of rRNA-processing complex regulated by nucleolar chaperon NVL2
| Project/Area Number |
24790095
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Meiji Pharmaceutical University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | プロテオーム / リボソーム生合成 / NVL2 / DOB/MTR4 / SPF30 / WDR74 |
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| Research Abstract |
AAA family proteins regulate the dissociation of molecular complexes by their ATPase activities. Nucleolar AAA-ATPase NVL2 regulates the biogenesis of 60S ribosome via the interaction with RNA helicase DOB1/MTR4. Therefore, it is suggested that there are dissociating factors regulated by NVL2 in DOB1-containing complex. However, the dissociating proteins are not yet to be identified. In this study, the experimental system that DOB1-interacting proteins could be comprehensively identified was first established by proteomics using fluorescent two-dimensional difference gel electrophoresis and mass spectrometric analysis. Using this system, SPF30 and WDR74 were identified as the dissociating-factors regulated by NVL2 from DOB1-containing complex.
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