Screening and evaluation for molecules involved in cholestrerol transport

2015 Fiscal Year Final Research Report

• Project/Area Number: 24790104
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Ishitsuka Reiko 国立研究開発法人理化学研究所, 小林脂質生物学研究室, 専任研究員 (60342747)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: コレステロール

Outline of Final Research Achievements

Cholesterol is a major component of lipids on plasma membranes and plays an important role on the formation of lipid rafts. The cholesterol content and distribution in cells is properly regulated. To examine know how intracellular cholesterol traffic and distribution are regulated, we screened chemical compounds involved in cholesterol traffic and distribution using a cholesterol-binding probe. We screened compounds that decrease the cholesterol probe-binding to the plasma membrane. We obtained compounds which inhibited cholesterol synthesis or cholesterol transport from the ER to the plasma membrane, or accumulated cholesterol in the late endosomes. We also got a compound which binds to liposomes and regulates the formation of the lipid domain.

Free Research Field

生物科学