2013 Fiscal Year Final Research Report
Development of enzyme-responsive anticancer agents and fluorescent probes targeting the biosynthesis of nucleotides
| Project/Area Number |
24790108
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 核酸の生合成 / カルボニル基のリン酸化 / ヌクレオチド / 酵素応答性分子 / イノシン / 蛍光標識化 |
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| Research Abstract |
In this study, we developed a novel method to synthesize chemically modified analogs of inosine 6-phosphates, which are observed in the biosynthesis of nucleotides. For this purpose, we applied a non-nucleophilic acidic activator, N-(cyanomethyl)dimethylammonium triflate (CMMT), that we developed recently, to the phosphitylation of carbonyl oxygen by the phosphoramidite method, which enabled us to synthesize inosine 6-phosphate diesters bearing various substituents on the phosphate. Furthermore, we evaluated the stability of the inosine 6-phosphate diesters to find that they were stable in neutral aqueous solutions. These molecules will be useful for biological and pharmaceutical studies of the biosynthesis of nucleotides.
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Research Products
(29 results)
