2013 Fiscal Year Final Research Report
Development of prodrug-siRNA activated by intracellular reducing environment
| Project/Area Number |
24790121
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Drug development chemistry
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| Research Institution | Osaka University of Pharmaceutical Sciences |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | RNA干渉 / siRNA / プロドラッグ / 機能性核酸 / 核酸化学 |
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| Research Abstract |
In this study, we developed 2'-O-methyldithiomethyl (MDTM)-RNA as a prodrug-RNA. The MDTM-RNAs showed high stability under fetal bovine serum, and were easily converted into native-RNAs under glutathione reducing condition found in the intracellular environment. In addition, the MDTM-RNAs showed enough affinity toward complementary RNA. The synthesis of MDTM-RNAs was effectively achieved by a post-synthetic approach. The MDTM-RNA might be an excellent candidate for RNA technologies such as RNA interference.
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Research Products
(11 results)
