2015 Fiscal Year Final Research Report
Combination of nucleic acid-loaded Bubble liposomes and high-intensity focused ultrasound for therapy of cerebrovascular accident
| Project/Area Number |
24790173
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 超音波 / リポソーム / ペプチド / 脳 |
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| Outline of Final Research Achievements |
We have previously developed polyethylene glycol-modified liposomes (Bubble liposomes [BLs]) that entrap ultrasound (US) contrast gas and can serve as both nucleic acids carriers and US contrast agents. In this study, we attempted to prepare nucleic acid-loaded and brain-targeting BLs modified with Angiopep-2 (Ang2) peptide. Ang2 is expected to be a useful ligand for the efficient delivery of nanocarriers to the brain. We showed that nucleic acid-loaded BLs modified with Ang2 accumulated in brain tissue after intravascular injection. We also confirmed that above-mentioned BLs could enhance the intensities of US imaging in brain tissue and the effects of pDNA transfection to the US exposed area of the brain. These results suggested that Ang2-BLs may be a useful therapeutic and diagnostic tool for cerebrovascular accident.
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| Free Research Field |
医歯薬学
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