2015 Fiscal Year Final Research Report
Analytical development for determination of isoprenoid compounds in human plasma and usefulness as a new target of anti-cancer drugs
| Project/Area Number |
24790175
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Medical pharmacy
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| Research Institution | Nigata University of Phermacy and Applied Life Sciences |
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Principal Investigator |
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| Research Collaborator |
YAMATO Susumu 新潟薬科大学, 薬学部, 教授 (60057370)
KIMURA Shinya 佐賀大学, 医学部, 教授 (80359794)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | ファルネシルピロリン酸 / LC-MS/MS / がん / イソプレノイド化合物 / ザラゴジン酸 / ヘッドスペースGC-FID / ポリフェノール |
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| Outline of Final Research Achievements |
Isoprenoids are intermediates of cholesterol biosynthesis, and also the precursors of Ras proteins related to cell proliferations. Recently, an inhibitory effect of bisphosphonate or statins on the biosynthesis of isoprenoid (especially, farnesyl pyrophosphate, FPP) has been noted as a target of an anti-cancer drug. In this study, we have developed a highly sensitive determination method of isoprenoids by LC-MS/MS and headspace GC-FID, and then applied to human hepatocellular carcinoma (HepG2) cells and human plasma samples.
Established analytical method was useful for functional estimation of bisphosphonate, zaragozic acid and polyphenols of hesperetin and naringenin by HepG2 cell culture. Furthermore, FPP concentration levels of lung cancer patients’ plasma were inclined to be lower than those of healthy volunteers’ plasma. Therefore, it was suggested that plasma FPP might be utilized as tumor marker.
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| Free Research Field |
臨床分析化学
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