Embryological analysis of mouse Multilineage-differentiating stress enduring (Muse) cells.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790188
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tohoku University
• Principal Investigator: WAKAO Shohei 東北大学, 医学(系)研究科(研究院), 助教 (80511948)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 多能性幹細胞

Outline of Final Research Achievements

Muse cells can be isolated from mesenchymal stem cells by FACS as cells positive for stage-specific embryonic antigen-3 (SSEA-3), a marker for undifferentiated human pluripotent stem cells. However, SSEA-3 is a glycolipid, it is difficult to embryological analysis. In order to search for the new marker, Muse cells were subjected to FACS analysis using various antibodies. From these results, it was suggested that Cripto-1 was a new marker in substitution for SSEA-3. Therefore, we constructed several lines of transgenic mice expressing GFP under the control of Cripto-1 promoter and subjected to histological analysis of these mice connective tissue. The GFP and SSEA-3 double positive cells were located sparsely in the connective tissue of the hypodermis and do not associated with particular structures such as blood vessels. These transgenic mice will be useful tool for future investigation of embryological analysis of Muse cells.

Free Research Field

幹細胞