2014 Fiscal Year Final Research Report
A role of V1a receptor in water/electrolytes metabolism.
| Project/Area Number |
24790222
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General physiology
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | V1aR / collecting duct / acidosis / dehydration |
|---|
| Outline of Final Research Achievements |
Arginine vasopressin (AVP) functions in the regulation of body fluid homeostasis, vasoconstriction, and ACTH release through vasopressin V1a, V1b (V3), and V2 receptors. We found that V1aR only localized in the intercalated cell (IC) type-A, type-B, non-A and non-B along the collecting duct (CD) in mouse kidney. The level of V1aR mRNA increased at the thick ascending limb (TAL) and IC of the CD in the inner stripe of the outer medulla after acid loading. V1aR may stimulate renal H＋ excretion and maintain the acid-base balance especially during the metabolic acidosis.
Furthermore, we have investigated the amount of AQP2 expression in WT and V1aR KO mice during euhydration and dehydration. The luminal expression of AQP2 significantly increased both in WT and V1aR KO during dehydration, although their levels of expression significantly decreased in V1aR KO mice. V1aR may contribute to water and electrolytes transport by maintaining the basal expression of AQP2 in kidney CD.
|
| Free Research Field |
腎生理
|
