2013 Fiscal Year Final Research Report
The mechanism of the function and physiological role of TASK channel in adrenaline secretion
| Project/Area Number |
24790228
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | TASKチャネル / エンドサイトーシス |
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| Research Abstract |
I investigated the molecular mechanism of regulation of TASK1 channels in endocrine cells. Pharmacological and molecular biological analyses showed that TASK1 channels are regulated by the clathrin-dependent endocytosis in NGF-stimulated adrenal medullary and PC12 cells. Mutation analysis of the TASK1 channel revealed that the dileucine motif was involved in at least part of the endocytosis. Furthermore, I showed that Src, PLC and PI3K are involved in NGF-induced endocytosis of TASK1 channels in PC12 cells. Additionally, the expression of TASK1 channels at the protein and mRNA levels was suppressed in PC12 cells treated with NGF for 2 weeks. These results indicate that NGF suppresses the expression of TASK1 channels in the plasma membrane via not only endocytosis but also the inhibition of gene transcription.
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Research Products
(5 results)
