2013 Fiscal Year Final Research Report
Calcineurin B homologous protein (CHP3) regulates cardiomyocyte hypertrophy via inhibition of GSK3beta phosphorylation
| Project/Area Number |
24790231
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
KOBAYASHI SOUSHI 独立行政法人国立循環器病研究センター, 研究所, 研究員 (50511531)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | CHP3 / 心筋細胞肥大 / GSK3β / 細胞内シグナル |
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| Research Abstract |
Calcineurin B homologous protein 3 (CHP3) is a 25-kDa calcium-binding protein mainly expressed in heart, but its function remains largely unknown. To understand the role of CHP3, we stably overexpressed CHP3 in fibroblast-derived cell line. When treated with insulin, there were significant decreases in phosphorylation level of GSK3beta in CHP3-overexpressed cells compared with that in control cells. Moreover, co-immunoprecipitation experiments demonstrated the interaction of CHP3 with GSK3beta. These results suggest that CHP3 serves as a novel regulatory factor for GSK3beta, which modulates cardiac hypertrophy.
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