2014 Fiscal Year Final Research Report
Analysis of relationship between neuregulin-cleavage and activity upon myelination by using visualization tecnique
| Project/Area Number |
24790262
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General pharmacology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ニューレグリン / ミエリン / ErbB / BACE1 |
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| Outline of Final Research Achievements |
Intercellular signaling through axonal Neuregulin-1 (NRG1) and ErbB receptor tyrosine kinase in Schwann cell or oligodendrocyte is important for myelination.
It is believed that cleavage of NRG1 to present its EGF-like growth factor to ErbB receptor is essential for ErbB activation. It is reported that cleavage of NRG1 by different enzymes result in different effects on myelination. But the molecular mechanism is remain to be elucidated.
In this study, we elucidated cleavage mechanism of NRG1 in detail and determined its cleavage sites. We successfully prepared anti-NRG1 cleavage-end specific antibody to detect and visualize its cleavage. We revealed the relationship between NRG1 cleavage and ErbB acitivation by establishing juxtacrine reconstitution system using cultured cells.
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| Free Research Field |
薬理学
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