Activity-dependent calcium signaling plays a role in development and maturation of cerebellar granule cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790300
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: National Cardiovascular Center Research Institute (2013-2014); Osaka Bioscience Institute (2012)
• Principal Investigator: OKAZAWA Makoto 独立行政法人国立循環器病研究センター, 研究所, 室長 (40414130)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 転写因子 / 神経成熟 / 膜電位

Outline of Final Research Achievements

In many developing neuronal cell types, the resting membrane potential shift from a depolarized state to a non-depolarized state during the early postnatal period. The shift of the resting membrane potential and the resulting inactivation of calcineurin are essential for activity-dependent maturation gene expression via Etv1 transcription factor and for morphological and electrophysiological maturation in cerebellar granule cells. In this study we analyzed the signaling mechanism of maturation gene expression and of immature gene suppression during cerebellar network formation. We found that Etv1 was regulated not only by activity-dependent calcium signaling but also by TrkB-ERK signaling and that Etv1 was associated with a transcriptional corepressor which might regulate immature gene expression.

Free Research Field

生理学