2013 Fiscal Year Final Research Report
The role of HSF4 in proteostasis
| Project/Area Number |
24790316
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TAKAKI Eiichi 山口大学, 医学(系)研究科(研究院), 学術研究員 (50525590)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 熱ショック応答 / 転写因子 |
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| Research Abstract |
Heat shock factors (HSFs) maintain protein homeostasis through regulating expression of heat shock proteins (HSPs), and non-HSP proteins in both control and stressed conditions. HSF family members consist of four members in mammals. Among them, HSF4 is required for the maintenance of lens, and HSF4 mutation is associated with human inherited cataract. Recently, it reveals that HSF4 plays an important role in adaption to stresses in other tissues or cell types. Uniquely, HSF4 constitutively forms a trimer, which can bind to DNA. However, it is unclear how the transcriptional activity of HSF4 is regulated in response to environmental or physiological signals. To find factors that regulate HSF4 activity, we performed immunoprecipitation analysis, followed by mass spectrometry. Furthermore, these factors were functionally screened. We identified a novel protein that promotes phosphorylation of HSF4. We propose a possible mechanism of HSF4 regulation by this novel factor.
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