Groundwork study for senescence indicator leading to diseases prevention and treatment with the aging

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790397
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology
• Principal Investigator: ITAKURA Yoko 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 研究員 (30582746)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 糖鎖 / 細胞老化 / 個体老化 / 再生 / レクチンマイクロアレイ

Outline of Final Research Achievements

We investigated cellular senescence and human aging-dependent glycan changes on human diploid fibroblasts derived from differently-aged skin and human cardiac cells derived from elderly patient with heart disease using lectin microarray. The glycan profiles in their early passage of elderly-derived fibroblasts were different from those of fetal-derived fibroblasts, especially α2-6sialylated glycan forms. Moreover, mesenchymal stem cell markers, such as CD-antigens including glycoproteins, in patient-cardiac cells were decreased in cellular senescence.
Therefore, it was suggested that the glycan change has the potential for senescence indicator. In the future, these findings will be helpful to evaluate the various multipotent stem cells.

Free Research Field

糖鎖生物学