DNA macromolecule as a vaccine antigen scaffold for organization of the antigens into repetitively ordered arrays

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790404
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Kagoshima University (2013); University of the Ryukyus (2012)
• Principal Investigator: MIYATA Takeshi 鹿児島大学, 農学部, 助教 (20448591)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ワクチン / マラリア / 伝搬阻止ワクチン / 三日熱マラリア原虫 / 抗原の反復整列 / アジュバント

Research Abstract

We have developed a new technology to enhance immune responses by generating complex which composed DNA-binding protein fused vaccine antigen and DNA (DBP-Ag/DNA). In this system, DNA was used as scaffold material, not genetic information. Vaccine efficacy of the DBP-Ag/DNA complex was evaluated using an ookinete surface protein of Plasmodium vivax, Pvs25. The DBP-Ag/DNA complex immunized mice were conferred a high immune response compared to Pvs25 alone immunized group. This system may be a promising approach for development of subunit vaccines against malaria.

Research Products

• [Journal Article] Expression of ovotransferrin enhances tolerance of yeast cells toward oxidative stress (2013)
  • Author(s): Ibrahim HR, Hozono A, Fukami M, Shaban MA, Miyata T
  • Journal Title: J. Agric Food Chem Volume: 61 Pages: 6358-65
  • DOI: 10.1021/jf401152e
  • Peer Reviewed
• [Journal Article] A bio-nanocapsule containing envelope (E) protein domain III of Japanese encephalitis virus (JEV) protects mice against lethal JEV infection (2013)
  • Author(s): Miyata T, Tafuku S, Harakuni T, Tadano M, Yoshimoto N, Iijima M, Matsuo H, Matsuzaki G, Kuroda S, Arakawa T
  • Journal Title: Microbiology and Immunology Volume: 57 Pages: 470-7
  • DOI: 10.1111/1348-0421.12055
  • Peer Reviewed
• [Journal Article] Expression and secretion of cholera toxin B subunit in lactobacilli (2013)
  • Author(s): Okuno T, Kashige N, Satho T, Irie K, Hiramatsu Y, Sharmin T, Fukumitsu Y, Uyeda S, Yamada S, Harakuni T, Miyata T, Arakawa T, Imoto M, Toda A, Nakashima Y, Miake F
  • Journal Title: Biol. Pharm. Bull Volume: 36 Pages: 952-8
  • DOI: 10.1248/bpb.b12-01021
  • Peer Reviewed
• [Journal Article] Physicochemically stable cholera toxin B subunit pentamer created by peripheral molecular constraints imposed by de novo-introduced intersubunit disulfide crosslinks (2012)
  • Author(s): Miyata T, Oshiro S, Harakuni T, Taira T, Matsuzaki G, Arakawa T
  • Journal Title: Vaccine Volume: 30 Pages: 4225-32
  • DOI: 10.1016/j.vaccine.2012.04.047
  • Peer Reviewed
• [Journal Article] Inhibitory effects of JNK on Aedes albopictus early larval development (2012)
  • Author(s): Satho T, Nagano Y, Eshita Y, Hisatomi Y, Sakata A, Miyata T, Kashige N, Miake F, Runtuwen RL, Fukushi S, Saijyo M, Kurane I, Morioka S, MizutaniT
  • Journal Title: Urban Pest Management Volume: 2 Pages: 7-13
  • Peer Reviewed
• [Presentation] The potential of recombinant ferritins as anti-tick vaccine against Haemaphysalis longicornis (2014)
  • Author(s): Remil Linggatong, Takeshi Miyata, Rika Umemiya-Shirafuji, Hiroki Maeda, Kodai Kusakisako, Masami Mochizuki, Kozo Fujisaki, Tetsuya Tanaka
  • Organizer: 第83回日本寄生虫学大会
  • Place of Presentation: 愛媛大学、愛媛
  • Year and Date: 20140327-28
• [Presentation] 三日熱マラリア伝搬阻止ワクチン候補抗原(Pvs25)の反復整列化とそのワクチン効果 (2014)
  • Author(s): 原國哲也、山田清太郎、宮田健、山口類、玉城志博、坪井敬文、Jetsumon Sattabongkot、橘真由美、鳥居本、新川武
  • Organizer: 第83回日本寄生虫学大会
  • Place of Presentation: 愛媛大学、愛媛
  • Year and Date: 20140327-28
• [Presentation] Induction of early protective immunity against pulmonary Mycobcterium tuberculosis infection in mice by combination of BCG priming vaccine and boosting mucosal vaccine with a recombinant mycobacterial antigen (2013)
  • Author(s): Masayuki Fukui, Masayuki Umemura, Takeshi Miyata, Tetsuya Harakuni, Takeshi Arakawa, Goro Matsuzaki
  • Organizer: 第42回日本免疫学会
  • Place of Presentation: 幕張メッセ、千葉
  • Year and Date: 20131211-13
• [Presentation] マラリアワクチン抗原搭載三部構成免疫賦活システム(TIPS)のマラリア伝搬阻止ワクチン機能解析 (2013)
  • Author(s): 玉城志博、宮田健、原國哲也、坪井敬文、Jetsumon Sattabongkot、橘真由美、鳥居本美、新川武
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール、長崎
  • Year and Date: 20131003-05
• [Presentation] コレラトキシンB鎖と三日熱マラリア伝搬阻止ワクチン抗原Pvs25の融合体構築とそのワクチン機能解析 (2013)
  • Author(s): 原國哲也、宮田健、坪井敬文、Jetsumon Sattabongkot、橘真由美、鳥居本美、新川武
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール、長崎
  • Year and Date: 20131003-05
• [Presentation] 日本脳炎ウイルス抗原、コイルドコイル5量体、免疫グロブリン結合ドメインから構成される三部構成複合体のワクチン機能解析 (2013)
  • Author(s): 山口類、新川武、宮田健、原國哲也、田福宣治、只野昌之
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール、長崎
  • Year and Date: 20131003-05
• [Presentation] リガンドの換装による三部構成免疫賦活システム(TIPS)の三日熱マラリア伝搬阻止ワクチン機能への影響 (2013)
  • Author(s): 山田清太郎、宮田健、原國哲也、坪井敬文、Jetsumon Sattabongkot、橘真由美、鳥居本美、新川武
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学、東京
  • Year and Date: 20130329-31
• [Presentation] Plasmodium yoelii MSP1の高分子量化による可溶性凝集体化とそのワクチン機能増強効果 (2013)
  • Author(s): 原國哲也、宮田健、山田清太郎、山口類、坪井敬文、Jetsumon Sattabongkot、橘真由美、鳥居本美、新川武
  • Organizer: 第82回日本寄生虫学会大会
  • Place of Presentation: 東京医科歯科大学、東京、発表
  • Year and Date: 20130329-31
• [Presentation] リガンド部分変更による三部構成免疫賦活システム(TIPS)の機能性向上の検討 (2012)
  • Author(s): 宮田健、原國哲也、田福宣治、坪井敬文、Jetsumon Sattabongkot、橘真由美、鳥居本美、只野昌之、新川武
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 20121117-18
• [Presentation] コレラトキシンB鎖の構造安定化に向けた分子改変 (2012)
  • Author(s): 原國哲也、宮田健、大城聡、平良東紀、新川武
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 20121117-18
• [Remarks]
  • URL: http://ace1.agri.kagoshima-u.ac.jp/agri0033/
• [Patent(Industrial Property Rights)] 薬物運搬体並びにこれを利用したアジュバントおよびワクチン (2013)
  • Inventor(s): 新川武、宮田健、松崎吾朗、坪井敬文
  • Industrial Property Rights Holder: 琉球大学
  • Industrial Property Rights Type: 米国特許
  • Industrial Property Number: 第8,580,274号
  • Filing Date: 2013-11-12
  • Overseas