2013 Fiscal Year Final Research Report
Investigation of the functions of gamma-glutamyltranspeptidease in Salmonella
| Project/Area Number |
24790419
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | サルモネラ / γーグルタミルトランスペプチダーゼ |
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| Research Abstract |
To understand the role of gamma-glutamyltranspeptidase (GGT) in Salmonella, I investigated whether GGT is contributed to the Salmonella virulence. Although I analyzed the functions of GGT using the ggt gene-disrupted mutant, I could not find the association with GGT and Salmonella virulence.
Salmonella was cultured at 20 C, GGT activity was elevated than that at 37 C. When I investigated the molecular mechanisms on GGT activity, I found that GGT activity in the hfq gene-disrupted mutant (hfq-mutant) was decreased compared with that of wild-type Salmonella. In addition, GGT activity in the hfq gene-complemented hfq-mutant was elevated compared with that of hfq-mutant. These results suggested that the RNA chaperone Hfq is involved in the GGT activity. Therefore, I speculated that production of GGT in Salmonella is the low temperature dependent manner and a novel non-coding small RNA in Salmonella should be associated with the exhibition of GGT activity.
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Research Products
(21 results)
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[Journal Article] Endoplasmic reticulum stress contributes to Helicobacter pylori VacA-induced apoptosis2013
Author(s)
Akazawa Y, Isomoto H, Matsushima K, Kanda T, Minami H, Yamaguchi N, Taura N, Shiozawa K, Ohnita K, Takeshima F, Nakano M, Moss J, Hirayama T, Nakao K
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Journal Title
PLoS One
Volume: 8
Pages: e82322
DOI
Peer Reviewed
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[Journal Article] Low-density lipoprotein receptor-related protein-1 (LRP1) mediates autophagy and apoptosis caused by Helicobacter pylori VacA2012
Author(s)
Yahiro K, Satoh M, Nakano M, Hisatsune J, Isomoto H, Sap J, Suzuki H, Nomura F, Noda M, Moss J, Hirayama T
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Journal Title
J Biol Chem
Volume: 287
Pages: 31104-15
DOI
Peer Reviewed
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[Presentation] Identification of Helicobacter pylori VacA in human lung and its effects on airway epithelial cells2013
Author(s)
Nakashima S, Kakugawa T, Harada T, Hara S, Sakamoto N, Nakano M, Ishimatsu Y, Isomoto H, Mukae H, Kurazono H, Hirayama T, Kohno S
Organizer
ERS Annual Congress 2013
Place of Presentation
Barcelona, Spain
Year and Date
20130907-11
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