Analysis of intracellular pathogenesis of Legionella in the host

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790425
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bacteriology (including Mycology)
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: ARASAKI Kohei 東京薬科大学, 生命科学部, 助教 (70609990)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: レジオネラ菌 / レジオネラエフェクター / 細胞内膜輸送 / 繋留因子

Research Abstract

Legionella pneumophila (L. pneumophila) is a pathogenic microbe that cause sever pneumonia. In L. pneumophila infection to the host cell, L. pneumophila hijack intracellular trafficking pathway and Legionella effector proteins that are secreted from the L. pneumophila to the host cell play a crucial role in this hijacking. In this study, I revealed that one of the Legionella effector protein, DrrA manipulates exocyst complex (host protein complex) that is implicated in the intracellular trafficking and manipulation of exocyst complex by DrrA is required for the effective intracellular growth of L. pneumophila.

Research Products

• [Journal Article] A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex (2013)
  • Author(s): Arasaki K, Takagi D, Furuno A, Sohda M, Misumi Y, Wakana Y, Inoue H, Tagaya M
  • Journal Title: Mol Biol Cell Volume: 24 Pages: 2907-17
  • DOI: 10.1091/mbc.E13-01-0014.
• [Journal Article] Contribution of the long form of syntaxin 5 to the organization of the endoplasmic reticulum (2012)
  • Author(s): Miyazaki, K., Wakana, Y., Noda, C., Arasaki, K., Furuno, A. and Tagaya, M
  • Journal Title: J Cell Sci Volume: 125 Pages: 5658-66
  • DOI: 10.1242/jcs.105304.
• [Presentation] レジオネラエフェクターLidAを通じたレジオネラ感染に関与するRabタンパク質の網羅的探索 (2013)
  • Author(s): 川端美緒、新崎恒平
  • Organizer: 第86回日本細菌学会総会ポスター及びワークショップ
  • Place of Presentation: 千葉
  • Year and Date: 20130300
• [Presentation] レジオネラエフェクターであるDrrAはER小胞とLCV膜との融合を促進する (2012)
  • Author(s): 新崎恒平、川端美緒、Derek Toomre、多賀谷光男、Craig Roy
  • Organizer: 第85回日本生化学大会シンポジウム
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] syntaxin17はmitochondria-associated membrane (MAM)に局在し、脂肪滴の形成に関与する (2012)
  • Author(s): 清水浩顕、茂刈寛史、西田直貴、新崎恒平、多賀谷光男
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: 福岡 ポスター
  • Year and Date: 20121200
• [Presentation] VIMPは微小管結合タンパク質との相互作用を介して小胞体の形態を調整する (2012)
  • Author(s): 野田睦乃、松下充、新崎恒平、多賀谷光男
  • Organizer: 第85回日本生化学大会
  • Place of Presentation: 福岡 ポスター
  • Year and Date: 20121200
• [Presentation] 小胞体とミトコンドリアの接触部位におけるsyntaxin 17の役割 (2012)
  • Author(s): 多賀谷光男、新崎恒平
  • Organizer: 第86回日本生化学大会シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 20120900
• [Book] オルガネラ接触部位:多様な機能と疾患、実験医学、Vol 31、No.11 (2013)
  • Author(s): 多賀谷光男、谷佳津子、新崎恒平
  • Total Pages: 1791-1797