2014 Fiscal Year Final Research Report
Isolation culture of hardly cultivable bacteria from human feces using fecal mediums and bacterial genome and metagenome analysis
| Project/Area Number |
24790429
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 難培養細菌 / 未知培養細菌 / 腸内細菌叢 / メタゲノム解析 / ゲノム解析 / 培地 |
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| Outline of Final Research Achievements |
In human intestines, there are many unknown, hardly cultivable and previously uncultivated bacteria. The aim of this study was to cultivate unknown and/or hardly cultivable bacteria in conditions close to the environment of original habitat, followed by to analyze the characteristics of those bacterial genome. This study showed that the bacteria in human gut were able to grow in medium made from human feces. Moreover, the bacteria of different types were detected by the fecal mediums which were sterilized with different methods. The 16S rRNA gene metagenomic analysis revealed that some of DNA sequences, which were isolated from bacteria grown in fecal mediums, did not show high identity to known bacterial rRNA genes in existing databases. These data suggested that unknown and/or hardly cultivable bacteria of human gut might be cultured from human fecal mediums.
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| Free Research Field |
細菌学、ゲノム生物学、情報生物学
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