2013 Fiscal Year Final Research Report
Analysis of aminoglycoside-resistant genes in highly multidrug-resistant Pseudomonas aeruginosa
| Project/Area Number |
24790432
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | 独立行政法人国立国際医療研究センター |
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Principal Investigator |
TADA Tatsuya 独立行政法人国立国際医療研究センター, その他部局等, その他 (00624644)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 多剤耐性緑膿菌 / 分子疫学 / 薬剤耐性因子 / アミノグリコシド / β-ラクタマーゼ |
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| Research Abstract |
We performed a molecular epidemiolygical study of 300 clinical isolates of Pseudomonas aeruginosa in 2011 in Japan. Of them, we found a novel aminoglycoside 6'-N-acetyltransferase gene, aac(6')-Iaj.The encoded protein, AAC(6')-Iaj, was found to consist of 184 amino acids, with 70% identity to AAC(6')-Ia. Escherichia coli transformed with a plasmid containing the aac(6')-Iaj gene acquired resistance to all aminoglycosides tested except gentamicin. Of note, aac(6')-Iaj contributed to the resistance to arbekacin.
In 2012, we found two novel IMP-type metallo-beta-lactamase variants, IMP-43 and IMP-44, which were identified in multidrug-resistant Pseudomonas aeruginosa isolates obtained in medical settings in Japan. These two variants had more efficient catalytic activities against all carbapenems tested, including dripenem, imipenem, meropenem and panipenem.
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Research Products
(12 results)
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[Journal Article] Emergence of 16S rRNA methylase-producing Acinetobacter baumannii and Pseudomonas aeruginosa isolates in Vietnam2013
Author(s)
Tatsuya Tada, Tohru Miyoshi-Akiyama, Yasuyuki Kato, Norio Ohmagari, Nozomi Takeshita, Nguyen V. Hung, Doan M. Phuong, Truong A. Thu, Nguyen G. Binh, Nguyen Q. Anh, Tran T. T. Nga, Pham H. Truong, Phan T. Xuan, Le T. A. Thu, Nguyen T. Son, Teruo Kirikae
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Journal Title
BMC Infect Dis
Volume: 13
Pages: 251
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