Development of a rapid and sensitive cell-fusion-based phenotypic HIV-1 tropism assay

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790437
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: The University of Tokyo
• Principal Investigator: HOSOYA Noriaki 東京大学, 医科学研究所, 特任助教 (30568928)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: HIV / 細胞指向性 / Envタンパク質 / ２重分割タンパク質 / 感染症 / ウイルス

Research Abstract

We developed a novel HIV-1 phenotypic tropism assay (DSP-Pheno) based on the cell fusion. We employed dual split protein (DSP) composed of split green fluorescent protein (GFP) and split renilla luciferase (RL) as a marker for cell fusion. Equipped with two-way reporter system, RL and GFP, DSP-Pheno is a sensitive test with short turnaround time. Although maintenance of cell lines and laboratory equipment is necessary, it provides a safe assay system without infectious viruses. With further validation against other conventional analyses, DSP-Pheno may prove to be a useful laboratory tool.

Research Products

• [Journal Article] Development of a rapid cell-fusion-based phenotypic HIV-1 tropism assay (2013)
  • Author(s): Teeranaipong P, Hosoya N, Kawana-Tachikawa A, Fujii T, Koibuchi T, Nakamura H, Koga M, Kondo N, Gao GF, Hoshino H, Matsuda Z, Iwamoto A.
  • Journal Title: Journal of the International AIDS Society Volume: 18巻 Pages: 18723-18732
  • DOI: 10.7448/IAS.16.1.18723
  • Peer Reviewed
• [Journal Article] Significant reductions in Gag-protease-mediated HIV-1 replication capacity during the course of the epidemic in Japan (2013)
  • Author(s): Nomura S, Hosoya N, Brumme ZL, Brockman MA, Kikuchi T, Koga M, Nakamura H, Koibuchi T, Fujii T, Carlson JM, Heckerman D, Kawana-Tachikawa A, Iwamoto A, Miura T.
  • Journal Title: Journal of Virology Volume: 87巻 Pages: 1465-1476
  • DOI: 10.1128/JVI.02122-12
  • Peer Reviewed
• [Journal Article] Improved neutralizing antibody response in the second season after a single dose of pandemic (H1N1) 2009 influenza vaccine in HIV-1-positive adults (2012)
  • Author(s): Kikuchi T, Iwatsuki-Horimoto K, Adachi E, Koga M, Nakamura H, Hosoya N, Kawana-Tachikawa A, Koibuchi T, Miura T, Fujii T, Kawaoka Y, Iwamoto A.
  • Journal Title: Vaccine Volume: 30巻 Pages: 3819-3823
  • DOI: 10.1016/j.vaccine.2012.03.083
  • Peer Reviewed
• [Presentation] 細胞膜融合と二重分割タンパク質(Dual Split Protein : DSP)を用いたHIV-1細胞指向性試験の開発 (2013)
  • Author(s): 細谷紀彰、Phairote Teeranaipong、立川(川名)愛、鯉渕智彦、古賀道子、中村仁美、近藤直幸、星野洪郎、松田前衛、岩本愛吉
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 20131110-12
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: 7th IAS Conference on HIV Pathogenesis, Treatment and Prevention
  • Place of Presentation: マレーシア、クアラルンプール
  • Year and Date: 20130630-0703
• [Presentation] Phenotypic determination of HIV-1 envelope tropism using Dual Split Protein (DSP) mediated cell fusion assay system (2013)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Stucki Heinz, Yoshimi Kashi, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: Asian Research Forum on Emerging and Reemerging Infections–2013
  • Place of Presentation: 東京都文京区
  • Year and Date: 20130123-24
• [Presentation] A novel phenotypic HIV-1 tropism assay (2012)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: The 17th Chinese Biopharmaceutical Association Annual Conference
  • Place of Presentation: 中国青島市
  • Year and Date: 20120702-03
• [Presentation] Development of a HIV-1 Phenotypic Tropism Assay Using Dual Split Protein (DSP)-Mediated Membrane Fusion Detection System (2012)
  • Author(s): Noriaki Hosoya, Phairote Teeranaipong, Ai Kawana-Tachikawa, Naoyuki Kondo, Hiroo Hoshino, Zene Matsuda, Aikichi Iwamoto
  • Organizer: 1st Asian Conference on Hepatitis B and C, HIV and Influenza
  • Place of Presentation: 中国北京市
  • Year and Date: 20120518-19
• [Remarks]
  • URL: http://www.idimsut.jp