2013 Fiscal Year Final Research Report
Role of poly-proline motif in HIV-2 Vpx expression.
| Project/Area Number |
24790443
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Virology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
MIYAZAKI Yasuyuki 徳島大学, 大学院ヘルスバイオサイエンス研究部, 助教 (70607233)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ＨＩＶ |
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| Research Abstract |
Human immunodeficiency virus type 2 (HIV-2) carries an accessory protein Vpx that is important for viral replication in natural target cells. In its C-terminal region, there is a highly conserved poly-proline motif (PPM) consisting of seven consecutive prolines. We have shown that PPM is critical for Vpx expression and viral infectivity. To elucidate the molecular basis underlying this observation, we analysed the expression of Vpx proteins with various PPM mutations by in vivo and in vitro systems. We found that the number and position of consecutive prolines in PPM are important for Vpx expression, and demonstrated that PPM is essential for efficient Vpx translation. Finally, we examined the Vpx of simian immunodeficiency virus from rhesus monkeys (SIVmac), which also has seven consecutive prolines, for PPM-dependent expression. A multi-substitution mutation in the PPM markedly reduced the expression level of SIVmac Vpx.
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Research Products
(10 results)
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[Journal Article] Poly-proline motif in HIV-2 Vpx is critical for its efficient translation2014
Author(s)
Miyake Ariko, Fujita Mikako, Fujino Haruka, Koga Ryoko, Kawamura Sogo, Otsuka Masami, Ode Hiroaki, Iwatani Yasumasa, Sakai Yosuke, Doi Naoya Nomaguchi Masako, Adachi Akio, Miyazaki Yasuyuki
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Journal Title
Journal of Genneral Virology
Volume: 95(Pt 1)
Pages: 179-189
DOI
Peer Reviewed
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