Role of T-cells on protective immune response to rotavirus infection.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790454
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: FUJII Yoshiki 国立感染症研究所, その他部局等, 研究員 (40518122)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ロタウイルス / マウス / T細胞受容体 / 次世代シーケンス

Outline of Final Research Achievements

T-cell responses on murine rotavirus infection were analyzed in detail by using the mouse model. Murine rotavirus EW strain was inoculated orally to Balb/c mice. The maximum T-cell proliferation was observed at 10 days after infection. The nucleotide sequences of T-cell receptors (TCRs) expressed in ileum at 10 days after rotavirus infection were comprehensively analyzed by using next-generation sequencer. Then, it was found that rotavirus-specific TCRs accumulated on rotavirus-infected ileum and the sequences could be identified. In future studies, we will analyze the reactivity of these T-cells, which enables us to understand the mechanisms of protective immunity and cross-reactivity among virus strains.

Free Research Field

ウイルス学