2013 Fiscal Year Annual Research Report
小腸自然免疫のリンパ系細胞と肥満細胞の機能的な相互関係の解明
| Project/Area Number |
24790474
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| Research Institution | Osaka University |
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Principal Investigator |
李 英愛 大阪大学, 免疫学フロンテイア研究センター, 特任研究員(常勤) (60610681)
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| Keywords | Innate lymphoid cells / Small intestine / IL-22 / α-defensins / Mast cell progenitors |
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| Research Abstract |
Small intestinal innate lymphoid cells (ILCs) regulate intestinal epithelial cell homeostasis and help to prevent pathogenic bacterial infections by producing IL-22. In a microarray analysis comparing small intestinal ILCs (Lin-c-Kit+Sca-1-cells) with non-ILCs (Lin-c-Kit-Sca-1- cells), we found that Lin-c-Kit+Sca-1- cells highly expressed the mRNAs for Il22, antimicrobial peptides, mast cell proteases, and Rorc. We then subdivided the Lin-c-Kit+Sca-1- cells into three groups―Lin-c-Kit+NKp46-CD4-, CD4+ LTi-like cells, and NKp46+ ILC22 cells―and showed that the Lin-c-Kit+NKp46-CD4- cells produced the highest level of IL-22 protein after IL-1β, IL-23, or IL-1β and IL-23 stimulation. We also showed that the majority of the Lin-c-Kit+NKp46-CD4- population was IL-7R+CD34-β7int cells, and IL-7R- cells could be divided into three subsets (CD34+β7int, CD34-β7int, and CD34intβ7hi cells). The IL-7R+CD34-β7int cells strongly expressed the transcripts for Il17f and Il22 after costimulation with IL-1β and IL-23. The IL-7R-CD34+β7int and IL-7R-CD34intβ7hi cells differentiated almost entirely into mast cells, whereas the IL-7R-CD34-β7int cells highly expressed a-defensins and showed no differentiation. Taken together, these findings indicate that the IL-7R-CD34+β7int and IL-7R-CD34intβ7hi populations are mast cell progenitors, and the IL-7R+CD34-β7int (CD4- LTi-like cells) and IL-7R-CD34-β7int populations within Lin-c-Kit+NKp46-CD4- cells may control intestinal homeostasis and provide intestinal protection by producing high levels of IL-22 and α-defensins, respectively.
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Research Products
(3 results)
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[Journal Article] Role of Mouse and Human Autophagy Proteins in IFN-r-Induced Cell-Autonomous Responses against Toxoplasma gondii2014
Author(s)
Jun Ohshima, Youngae Lee, Miwa Sasai, Tatsuya Saitoh, Ji Su Ma, Naganori Kamiyama, Yoshiharu Matsuura, Suh Pann-Ghill, Mikako Hayashi, Shigeyuki Ebisu, Kiyoshi Takeda, Shizuo Akira, and Masahiro Yamamoto
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Journal Title
The Journal of Immunology
Volume: 192
Pages: 3328-3335
DOI
Peer Reviewed
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