2013 Fiscal Year Final Research Report
Synthesis of [18F]labelled proteins using cell-free system
| Project/Area Number |
24790527
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied pharmacology
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| Research Institution | Tohoku University |
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Principal Investigator |
SHIBUYA Katsuhiko 東北大学, 医学(系)研究科(研究院), 教育研究支援者 (30596268)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | イメージング |
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| Research Abstract |
First, we synthesized [19F]-interleukin-8 (IL-8) which contained non-labelled fluoroproline instead of proline using the cell-free protein synthesis system. We confirmed that pure and abundant [19F]-IL-8 could be obtained. The binding affinity of [19F]IlL-8 to IL8 receptors was retained. Next, we synthesized [18F]IL-8 using [18F]proline. After ion-exchanged chromatography, we could obtained [18F]IL-8 which had 91% of radiochemical purity in a short time.
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[Journal Article] Mechanism of the histamine H3 receptor - mediated increase in exploratory locomotor activity and anxiety - like behaviours in mice2014
Author(s)
Mohsen A, Yoshikawa T, Miura Y, Nakamura T, Naganuma F, Shibuya K , Iida T, Harada R, Okamura N, Watanabe T, Yanai K
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Journal Title
Neuropharmacology
Volume: 81
Pages: 188-194
DOI
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