Elucidation and evasion of the immunogenicity induced by the repeated administration of the PEGylated nanocarrier

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790530
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied pharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: TOH Kazuko 東京大学, 医学(系)研究科(研究院), 研究員 (90342732)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: ドラッグデリバリーシステム / ポリエチレングリコール / ABC現象 / 生体内共焦点顕微鏡

Outline of Final Research Achievements

In drug delivery system (DDS), the modification of nanocarriers with polyethylene glycol (PEGylated nanocarrier) is commonly used technique to improve blood circulation time. However, it is reported that repeated injection of PEGylated carrier cause ‘accelerated blood clearance (ABC)’ phenomenon in animal experiments. The ABC phenomenon is one of the barriers in the DDS development. In this study, the convenient evaluation method using in vivo confocal microscopy was established. After the investigation of various nanocarrier, the structure avoiding the ABC phenomenon was examined. The evaluation method of siRNA intact ratio including the nanocarrier and the inhibition method of photobleaching in case of long time measurement were also established.

Free Research Field

ドラッグデリバリーシステム