Basic investigation of detection targeted at resistant-factors of drug-resistant microorganisms

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790551
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Nagoya University
• Principal Investigator: KEIKO Yamada 名古屋大学, 医学(系)研究科(研究院), 助教 (00402561)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 薬剤耐性 / 検出 / 抗体 / 感染症 / 検査 / 迅速

Outline of Final Research Achievements

In this study, we aimed to developed specific antibodies against resistant-factor for rapid detection of drug-resistant microorganisms. In this study, target molecules are key proteins of drug-resistance, 1) PBP2a of methicillin resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (MRCNS), 2) IMP-1 of carbapenem-resistant bacteria, and 3) oseltamivir- and peramivir-resistant type neuraminidase of influenza virus. I have prepared specific antibodies for them, and they showed the specificity with recombinant proteins and resistant/susceptible microorganisms. I constructed immunochromatography assay system using specific anti-PBP2a chicken IgY. It was revealed that it could detected MRSA and MRCNS directly from mimicked blood-cultured bottle in about 3 hours.

Free Research Field

病態検査学