Application to laboratory procedure based on the multistep tumorigenic events in adult T cell leukemia/lymphoma (ATLL)

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790553
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Okayama University
• Principal Investigator: SATO Hiaki 岡山大学, 保健学研究科, 助教 (70362960)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 成人T細胞白血病/リンパ腫（ATLL） / SHP1

Outline of Final Research Achievements

We analyzed to clarify the multistep tumorigenic events in adult T cell leukemia/lymphoma (ATLL) by focusing on SHP1. SHP1 is a nonreceptor type protein-tyrosine phosphatase, which acts as a negative regulator in hematopoietic cells. In immunostaining, the expression of SHP1 and tumor-related genes decreased in various ATLL-related cell lines. By performing methylation specific PCR (MSP) assay, we detected aberrant methylation of SHP1 and these genes. Moreover, we identified specific aberrant DNA methylation profile in ATLL-related cell lines. Our findings demonstrate that the presence of aberrant methylation is deeply involved in the development and progression of ATLL in multistep leukemogenesis.

Free Research Field

病理検査学