2014 Fiscal Year Final Research Report
Establishment of a serodiagnosis method and a serotyping method using VirB5 and BepA for cat scratch disease
| Project/Area Number |
24790554
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 猫ひっかき病 / Cat scratch disease / Bartonella henselae / VirB5 / ELISA |
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| Outline of Final Research Achievements |
In this study, we analyzed the seroreactivities of patients with cat scratch disease (CSD) and of healthy subjects against the VirB5 and BepA subtypes of Bartonella henselae. We developed an ELISA for measurement of anti-VirB5 antibody levels and a serotyping method for the VirB5 subtype. The sensitivity and specificity of the ELISA relative to an indirect fluorescence antibody (IFA) assay were 72.6% and 99.0%, respectively. For patients with positive results for anti-VirB5 antibodies, the serotyping method could identify the VirB5 subtype as being the strain causing infection. The VirB5 ELISA and the serotyping method for the VirB5 subtype are expected to be useful tools for molecular epidemiological studies of B. henselae infection.
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| Free Research Field |
臨床微生物学
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