Supporting characters may go to the heart of amyloidogenesis: Amyloid associated protein world

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790557
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine
• Research Institution: Kumamoto University
• Principal Investigator: TASAKI MASAYOSHI 熊本大学, 生命科学研究部, 助教 (50613402)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: アミロイドーシス / プロテオミクス / アミロイド共存蛋白質 / 臨床検査医学

Research Abstract

It is well known that some co-localized proteins with amyloid fibrils, which are called amyloid associated proteins, may play important roles in amyloidosis. Then, we tried to pick up the strong candidates as early diagnostic markers and/or therapeutic molecules of amyloidosis in this study. As the results of our studies, we could detect some strong candidates, such as clusterin and vitronectin. Serum clusterin levels of familial amyloidotic polyneuropathy (FAP) patients was significantly higher than that of healthy volunteers. Moreover, significant higher correlation between vitronectin and TTR deposition was observed in the cardiac tissues of FAP patients.

Research Products

• [Journal Article] Protein Phosphatase 6 Controls BCR-Induced Apoptosis of WEHI-231 Cells by Regulating Ubiquitination of Bcl-xL (2014)
  • Author(s): Kajihara R, Sakamoto H, Tanabe K, Takemoto K, Tasaki M, Ando Y, Inui S
  • Journal Title: J Immunol Volume: (in press)
  • Peer Reviewed
• [Journal Article] SIRT7 Controls Hepatic Lipid Metabolism by Regulating the Ubiquitin-Proteasome Pathway (2014)
  • Author(s): Yoshizawa T, Karim MF, Sato Y, Senokuchi T, Miyata K, Fukuda T, Go C, Tasaki M, Uchimura K, Kadomatsu T, Tian Z, Smolka C, Sawa T, Takeya M, Tomizawa K, Ando Y, Araki E, Akaike T, Braun T, Oike Y, Bober E, Yamagata K
  • Journal Title: Cell Metab Volume: 19 Pages: 712-21
  • Peer Reviewed
• [Journal Article] Generation of familial amyloidotic polyneuropathy-specific induced pluripotent stem cells (2014)
  • Author(s): Isono K, Jono H, Ohya Y, Shiraki N, Yamazoe T, Sugasaki A, Era T, Fusaki N, Tasaki M, Ueda M, Shinriki S, Inomata Y, Kume S, Ando Y
  • Journal Title: Stem Cell Res Volume: 12 Pages: 574-83
  • Peer Reviewed
• [Journal Article] パラフィン包埋ホルマリン固定組織切片を利用したLMD-LC-MS/MSシステムによる新たなアミロイドーシス診断法の構築 (2014)
  • Author(s): 田崎雅義、大林光念、植田光晴、安東由喜雄
  • Journal Title: 臨床病理 Volume: 62 Pages: 291-6
• [Journal Article] Usefulness of plasma B-type natriuretic peptide as a prognostic marker of cardiac function in senile systemic amyloidosis and in familial amyloidotic polyneuropathy (2013)
  • Author(s): Usuku H, Obayashi K, Shono M, Oshima T, Tasaki M, Yasuda H, Ogawa H, Ando Y
  • Journal Title: Amyloid Volume: 20 Pages: 251-5
• [Journal Article] Effect of age and sex differences on wild-type transthyretin amyloid formation in familial amyloidotic polyneuropathy : a proteomic approach (2013)
  • Author(s): Tasaki M, Ueda M, Obayashi K, Koike H, Kitagawa K, Ogi Y, Jono H, Su Y, Suenaga G, Oshima T, Misumi Y, Yoshida M, Yamashita T, Sobue G, Ando Y
  • Journal Title: Int J Cardiol Volume: 170 Pages: 69-74
  • Peer Reviewed
• [Journal Article] Changes in pathological and biochemical findings of systemic tissue sites in familial amyloid polyneuropathy more than 10 years after liver transplantation (2013)
  • Author(s): Oshima T, Kawahara S, Ueda M, Kawakami Y, Tanaka R, Okazaki T, Misumi Y, Obayashi K, Yamashita T, Ohya Y, Ihse E, Shinriki S, Tasaki M, Jono H, Asonuma K, Inomata Y, Westermark P, Ando Y
  • Journal Title: J Neurol Neurosurg Psychiatry Volume: (in press)
  • Peer Reviewed
• [Journal Article] Dominant cardiac type of familial amyloidotic polyneuropathy associated with a novel transthyretin variant Thr59Arg (2013)
  • Author(s): Obayashi K, Misumi Y, Watanabe T, Ando T, Akagami T, Tasaki M, Shinriki S, Ueda M, Yamashita T, Shinichi H, Ando Y
  • Journal Title: Muscle Nerve Volume: 48 Pages: 839-841
  • Peer Reviewed
• [Journal Article] Amyloid fibrils containing fragmented ATTR may be the standard fibril composition in ATTR amyloidosis (2013)
  • Author(s): Ihse E, Rapezzi C, Merlini G, Benson M, Ando Y, Suhr O, Ikeda SI, Lavatelli F, Obici L, Quarta C, Leone O, Jono H, Ueda M, Lorenzini M, Liepnieks J, Ohshima T, Tasaki M, Yamashita T, Westermark P
  • Journal Title: Amyloid Volume: 20 Pages: 142-150
  • Peer Reviewed
• [Journal Article] Impact of antibodies against amyloidogenic transthyretin (ATTR) on phenotypes of patients with familial amyloidotic polyneuropathy (FAP) ATTR Valine30Methionine (2013)
  • Author(s): Obayashi K, Tasaki M, Jono H, Ueda M, Shinriki S, Misumi Y, Yamashita T, Oshima T, Nakamura T, Ikemizu S, Anan I, Suhr O, Ando Y
  • Journal Title: Clin Chim Acta Volume: 419 Pages: 127-131
  • Peer Reviewed
• [Journal Article] Novel function of transthyretin in pancreatic alpha cells (2012)
  • Author(s): Su Y, Jono H, Misumi Y, Senokuchi T, Guo J, Ueda M, Shinriki S, Tasaki M, Shono M, Obayashi K, Yamagata K, Araki E, Ando Y
  • Journal Title: FEBS Lett Volume: 586 Pages: 4215-4222
  • Peer Reviewed
• [Journal Article] The occurrence of islet amyloid polypeptide amyloidosis in Japanese subjects (2012)
  • Author(s): Su Y, Misumi Y, Ueda M, Shono M, Tasaki M, Guo J, Jono H, Obayashi K, Senokuchi T, Yamagata K, Ando Y
  • Journal Title: Pancreas Volume: 41 Pages: 971-973
  • Peer Reviewed
• [Journal Article] Aged vervet monkeys developing transthyretin amyloidosis with the human disease-causing Ile122 allele : A valid pathological model of the human disease (2012)
  • Author(s): Ueda M, Ageyama N, Nakamura S, Nakamura M, Chambers J, Misumi Y, Mizuguchi M, Shinriki S, Kawahara S, Tasaki M, Jono H, Obayashi K, Sasaki E, Une Y, Ando Y
  • Journal Title: Lab Invest Volume: 92 Pages: 474-484
  • Peer Reviewed
• [Journal Article] Potential use of glucuronylglucosyl-β-cyclodextrin as a novel therapeutic tool for familial amyloidotic polyneuropathy (2012)
  • Author(s): Jono H, Anno T, Motoyama K, Misumi Y, Tasaki M, Oshima T, Mori Y, Mizuguchi M, Ueda M, Shinriki S, Shono M, Obayashi K, Arima H, Ando Y
  • Journal Title: Amyloid Volume: 19 Pages: 50-2
  • Peer Reviewed
• [Presentation] アミロイドーシス診断構築事業の2年間の取り組み : 血清および組織プロテオミクス解析法を駆使した診断システムの貢献度 (2014)
  • Author(s): 田崎雅義、大林光念、久原春代、植田光晴、荻泰裕、北川敬資、大隈雅紀、池田勝義、安東由喜雄
  • Organizer: 第25回日本臨床化学会九州支部総会合同プログラム,第59回日本臨床検査医学会九州地方会
  • Place of Presentation: 熊本市医師会館,熊本
  • Year and Date: 2014-03-05
• [Presentation] Effect of age and sex differences on transthyretin amyloid formation in FAP (2013)
  • Author(s): Tasaki M, Ueda M, Obayashi K, Koike H, Kitagawa K, Ogi Y, Suenaga G, Sobue G, Ando Y
  • Place of Presentation: Rio de Janeiro, Brazil
  • Year and Date: 20131111-13
• [Presentation] TTR型家族性アミロイドポリニューロパチーにおける組織沈着TTRアミロイドのプロテオーム解析 (2013)
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  • Organizer: 第60回日本臨床検査医学会
  • Place of Presentation: 神戸国際会議場,神戸
  • Year and Date: 20131031-1103
• [Presentation] 組織マススペクトルメトリー解析法を用いた全身性アミロイドーシスの病型診断における前向き検討 (2013)
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  • Organizer: 第38回日本医用マススペクトル学会年会
  • Place of Presentation: 神戸市産業振興センター,神戸
  • Year and Date: 20130926-27
• [Presentation] アミロイド抽出法を改良した質量分析装置によるアミロイドーシスの診断 (2013)
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  • Organizer: 第53回日本臨床化学会年次学術集会シンポジウム
  • Place of Presentation: あわぎんホール,徳島
  • Year and Date: 20130830-0901
• [Presentation] 組織プロテオーム解析による新規アミロイドーシス診断法の開発 (2012)
  • Author(s): 田崎雅義、植田光晴、三上紗弥香、韓美英、大嶋俊範、板東泰彦、神力悟、宇宿弘輝、城野博史、大林光念、安東由喜雄
  • Organizer: 第59回日本臨床検査医学会学術集会
  • Place of Presentation: 国立京都国際会館,京都
  • Year and Date: 20121129-1202
• [Presentation] (2012)
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  • Organizer: Typing of amyloidosis by proteome analysis 12th Meeting of the Asian Society of Clinical Pathology and Laboratory Medicine
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 20121129-1201
• [Presentation] LC-MS/ MSによる骨髄腫に伴う軽鎖沈着症の診断および病態解析 (2012)
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  • Organizer: 第52回日本臨床化学会年次学術集会
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• [Presentation] (2012)
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  • Organizer: RNAi therapy using cholesterol-conjugated siRNA for TTR-related ocular amyloidosis. 13th International Symposium on Amyloidosis
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  • Year and Date: 20120506-10