2014 Fiscal Year Final Research Report
Multiple analysis in colon of the stress-induced irritable bowel syndrome model
| Project/Area Number |
24790582
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Hoshi University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 過敏性腸症候群 / 炎症 / サイトカイン |
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| Outline of Final Research Achievements |
Diarrhea was induced by stress loaded with communication box in BALB/c mouse, although diarrhea was not caused in C57BL/6J mouse. Irritable bowel syndrome produces some symptoms similar to those of inflammatory bowel disease. In the present study, the gene expression of inflammatory cytokines in colon of the stress-loaded BALB/c mouse was examined. The expressions of various cytokines were attenuated by stress-loaded, although IL-4 and IL-17a gene expressions were increased by acute stress loaded, respectively. However, augmentation of these gene expressions was not correlated with development of diarrhea. On the other hand, gene expressions of TRPA1 and TRPV1 were increased by acute stress loaded. Furthermore, gene expression of cystic fibrosis transmembrane conductance regulator (CFTR) was increased by stress loaded. These results showed that the mechanism of stress-induced diarrhea is complicated in inflammation. Further studies are needed about involvement of TRPA1, TRPV1 and CFTR.
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| Free Research Field |
薬理学
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