2013 Fiscal Year Final Research Report
Analysis of the effects of oxidative stress on DNA methylation and the expression of active DNA demethylation related genes
| Project/Area Number |
24790600
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Hygiene
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
TAKUMI Shota 東京慈恵会医科大学, 医学部, 助教 (80570770)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 酸化ストレス / ＤＮＡ脱メチル化 / エピジェネティクス / ＤＮＡメチル化 |
|---|
| Research Abstract |
In this study, we investigated the effects of oxidative stress on DNA methylation and the expression of active DNA demethylation related genes. The results of this study showed that paraquat-induced oxidative stress increased the expression of Aid, which is DNA demethylation related gene. Furthermore, over-expression of Aid increased some genes expression. Taken together, these results may suggest that the oxidative stress-induced activation of Aid is involved in those genes activation through the DNA demethylation. Further analysis of promoter methylation of Aid-activated genes will help to understand the effect of oxidative stress on DNA methylation.
|
